TY  - JOUR
AU  - Pan, Mingfei
AU  - Hong, Liping
AU  - Yang, Jingying
AU  - Xie, Xiaoqian
AU  - Liu, Kaixin
AU  - Wang, Shuo
PY  - 2022
TI  - Fabrication and evaluation of a portable and reproducible quartz crystal microbalance immunochip for label-free detection of β-lactoglobulin allergen in milk products
JO  - Food Science and Human Wellness
SN  - 2097-0765
SP  - 1315
EP  - 1321
VL  - 11
IS  - 5
AB  - In this study, a label-free, portable and reproducible immunochip based on quartz crystal microbalance (QCM) was developed for the qualitative detection of β-lactoglobulin (β-LG), an allergen, in milk products. Experimental parameters in the fabrication and regeneration procedure such as pH of the coupling microenvironment, amount of anti-β-LG antibody and regeneration reagent were optimized in detail. Under optimal conditions, the proposed QCM immunochip exhibited good recognition of β-LG, with a calibration curve of ΔF = 12.877Cβ-LG0.4809 (R2 = 0.9982) and limit of detection of 0.04 μg/mL. Additionally, this portable QCM immunochip had good stability, high specificity, and no obvious cross-reaction to three other milk proteins (α-casein, α-lactalbumin, and lactoferrin). It could compete a qualitative measurement within 5 min, and could be reused at least ten times. In the β-LG analysis of actual milk samples, the developed QCM immunochip yielded reliable and accurate results, which correlated strongly with those from the standard HPLC method (R2 = 0.9969). Thus, the portable, stable, and reproducible QCM immunochip developed in this study allowed the rapid, cost-effectively and sensitively measure the β-LG in milk products.
UR  - https://doi.org/10.1016/j.fshw.2022.04.015
DO  - 10.1016/j.fshw.2022.04.015