TY - JOUR AU - Li, Xueling AU - Tian, Jing AU - Xu, Yan AU - Chen, Xiaomin AU - Liu, Zexi AU - Liang, Hui AU - Jin, Caihong AU - Cui, Daxiang PY - 2020 TI - Development of SARS-CoV-2 Isothermal Amplification Detection Kits JO - Nano Biomedicine and Engineering SN - 2097-3837 SP - 316 EP - 320 VL - 12 IS - 4 AB - The SARS-CoV-2 isothermal amplification detection kits based on loop-mediated isothermal amplification (LAMP) were developed and evaluated on three types samples of SARS-CoV-2. The kits included enzyme reaction mixtures and chromogenic agents. After the isothermal amplification reactions were completed, the reaction results were judged by using the chromogenic agents to determine whether SARS-CoV-2 exists in the samples to be tested. The detection kits have the advantages of convenient operation, fast detection speed and high sensitivity up to 1 copy of virus particles per reaction, which can speed up the detection speed of suspected cases, and avoid the missing detection problems caused by the low detection sensitivity. UR - https://doi.org/10.5101/nbe.v12i4.p316-320 DO - 10.5101/nbe.v12i4.p316-320