@article{Gazi2019, 
author = {Ayesha Siddiqua Gazi and Abbaraju Krishnasailaja},
title = {Preparation and Characterization of Methotrexate Loaded Polymeric Nanoparticles by Nanoprecipitation Technique},
year = {2019},
journal = {Nano Biomedicine and Engineering},
volume = {11},
number = {4},
pages = {351-360},
keywords = {Methotrexate, Polymeric nanoparticles, Nanoprecipitation technique},
url = {https://www.sciopen.com/article/10.5101/nbe.v11i4.p351-360},
doi = {10.5101/nbe.v11i4.p351-360},
abstract = {The aim of this study was to develop and characterize methotrexate-loaded polymeric nanoparticles by nanoprecipitation technique. Eudragit? S 100 and ethyl cellulose were employed to develop methotrexate-loaded nanoparticles by nanoprecipitation technique. Six different formulations (f1, f2, f3, f4, f5 and f6) were prepared with each polymer by varying the drug to polymer ratios (1:1, 1:1.5, 1:2, 1:3, 1.5:1 and 2:1). Dimethyl sulfoxide (DMSO) was used as a solvent and Tween? 20 as a surfactant. Among the six formulations of polymeric nanoparticles prepared by nanoprecipitation technique, F4 formulation was considered as a best formulation with each polymer. Based on comparison results of mean particle diameter, zeta potential, drug content and entrapment efficiency, Eudragit? S 100 was considered to be the most suitable polymer for preparation of methotrexate-loaded nanoparticles by nanoprecipitation technique. Based upon the evaluation studies, the best formulation was characterized for scanning electron microscopy (SEM), particle size, zeta potential and anti-cancer activity in MCF-7 cell line by MTT assay.}
}