TY  - JOUR
AU  - Gazi, Ayesha Siddiqua
AU  - Krishnasailaja, Abbaraju
PY  - 2019
TI  - Preparation and Characterization of Methotrexate Loaded Polymeric Nanoparticles by Nanoprecipitation Technique
JO  - Nano Biomedicine and Engineering
SN  - 2097-3837
SP  - 351
EP  - 360
VL  - 11
IS  - 4
AB  - The aim of this study was to develop and characterize methotrexate-loaded polymeric nanoparticles by nanoprecipitation technique. Eudragit? S 100 and ethyl cellulose were employed to develop methotrexate-loaded nanoparticles by nanoprecipitation technique. Six different formulations (f1, f2, f3, f4, f5 and f6) were prepared with each polymer by varying the drug to polymer ratios (1:1, 1:1.5, 1:2, 1:3, 1.5:1 and 2:1). Dimethyl sulfoxide (DMSO) was used as a solvent and Tween? 20 as a surfactant. Among the six formulations of polymeric nanoparticles prepared by nanoprecipitation technique, F4 formulation was considered as a best formulation with each polymer. Based on comparison results of mean particle diameter, zeta potential, drug content and entrapment efficiency, Eudragit? S 100 was considered to be the most suitable polymer for preparation of methotrexate-loaded nanoparticles by nanoprecipitation technique. Based upon the evaluation studies, the best formulation was characterized for scanning electron microscopy (SEM), particle size, zeta potential and anti-cancer activity in MCF-7 cell line by MTT assay.
UR  - https://doi.org/10.5101/nbe.v11i4.p351-360
DO  - 10.5101/nbe.v11i4.p351-360