@article{Xia2024, 
author = {Xuhan Xia and Chenxi Zhou and Yulin Zhu and Yi Dong and Qiang He and Mohammad Rizwan Khan and Yuanlong Chi and Rosa Busquets and Ruijie Deng and Yao Ren},
title = {Tb3+-nucleic acid probe-based label-free and rapid detection of mercury pollution in food},
year = {2024},
journal = {Food Science and Human Wellness},
volume = {13},
number = {2},
pages = {993-998},
keywords = {Mercury pollution, Food safety, Nucleic acid probe, Label-free, Terbium},
url = {https://www.sciopen.com/article/10.26599/FSHW.2022.9250085},
doi = {10.26599/FSHW.2022.9250085},
abstract = {Mercury is a threatening pollutant in food, herein, we developed a Tb3+-nucleic acid probe-based label-free assay for mix-and-read, rapid detection of mercury pollution. The assay utilized the feature of light-up fluorescence of terbium ions (Tb3+) via binding with single-strand DNA. Mercury ion, Hg2+ induced thymine (T)-rich DNA strand to form a double-strand structure (T-Hg2+-T), thus leading to fluorescence reduction. Based on the principle, Hg2+ can be quantified based on the fluorescence of Tb3+, the limit of detection was 0.068 9 μmol/L and the linear range was 0.1‒6.0 μmol/L. Due to the specificity of T-Hg2+-T artificial base pair, the assay could distinguish Hg2+ from other metal ions. The recovery rate was ranged in 98.71%‒101.34% for detecting mercury pollution in three food samples. The assay is low-cost, separation-free and mix-to-read, thus was a competitive tool for detection of mercury pollution to ensure food safety.}
}