@article{Liang2024, 
author = {Jingjing Liang and Meina Zhang and Xiaohan Li and Yuan Yue and Xiaowei Wang and Mengzhen Han and Tianli Yue and Zhouli Wang and Zhenpeng Gao},
title = {Structure and immunomodulatory activity of Lentinus edodes polysaccharides modified by probiotic fermentation},
year = {2024},
journal = {Food Science and Human Wellness},
volume = {13},
number = {1},
pages = {421-433},
keywords = {Lentinus edodes polysaccharide, Lactobacillus fermentum fermentation, Structural analysis, Immunoregulatory activity, Nuclear magnetic resonance},
url = {https://www.sciopen.com/article/10.26599/FSHW.2022.9250036},
doi = {10.26599/FSHW.2022.9250036},
abstract = {Plant-based fermentations provide an untapped source for novel biotechnological applications. In this study, a probiotic named Lactobacillus fermentum 21828 was introduced to ferment Lentinus edodes. Polysaccharides were extracted from fermented and non-fermented L. edodes and purified via DEAE-52 and Sephadex G-100. The components designated F-LEP-2a and NF-LEP-2a were analyzed by FT-IR, HPGPC, HPAEC, SEM, GC-MS and NMR. The results revealed that probiotic fermentation increased the molecular weight from 1.16 × 104 Da to 1.87 × 104 Da and altered the proportions of glucose, galactose and mannose, in which glucose increased from 45.94% to 48.16%. Methylation analysis and NMR spectra indicated that F-LEP-2a and NF-LEP-2a had similar linkage patterns. Furthermore, their immunomodulatory activities were evaluated with immunosuppressive mice. NF-LEP and F-LEP improved immune organ indices, immunoglobulin (IgG and IgM) and cytokines concentrations;restored the antioxidation capacity of liver;and maintained the balance of gut microbiota. F-LEP displayed better moderating effects on the spleen index, immunoglobulin, cytokines and the diversity of gut microbiota than NF-LEP (200, 400 mg/kg). Our study provides an efficient and environment-friendly way for the structural modification of polysaccharides, which helps to enhance their biological activity and promote their wide application in food, medicine and other fields.}
}