@article{Su2023, 
author = {Weiran Su and Junru Li and Chen Ji and Congshuo Chen and Yuzheng Wang and Huili Dai and Fengqin Li and Peifeng Liu},
title = {CRISPR/Cas systems for the detection of nucleic acid and non-nucleic acid targets},
year = {2023},
journal = {Nano Research},
volume = {16},
number = {7},
pages = {9940-9953},
keywords = {clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas), detection, nucleic acid targets, non-nucleic acid targets},
url = {https://www.sciopen.com/article/10.1007/s12274-023-5567-4},
doi = {10.1007/s12274-023-5567-4},
abstract = {Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems are becoming powerful tools for disease biomarkers detection. Due to the specific recognition, cis-cleavage and nonspecific trans-cleavage capabilities, CRISPR/Cas systems have implemented the detection of nucleic acid targets (DNA and RNA) as well as non-nucleic acid targets (e.g., proteins, exosomes, cells, and small molecules). In this review, we first summarize the principles and characteristics of various CRISPR/Cas systems, including CRISPR/Cas9, Cas12, Cas13 and Cas14 systems. Then, various types of applications of CRISPR/Cas systems used in detecting nucleic and non-nucleic acid targets are introduced emphatically. Finally, the prospects and challenges of their applications in biosensing are discussed.}
}