@article{Li2023, 
author = {Chenbiao Li and Peifang Chen and Xiaoyuan Ma and Xichi Lin and Shan Xu and Sobia Niazi and Zhouping Wang},
title = {Real-time in situ observation of P53-mediated cascade activation of apoptotic pathways with nucleic acid multicolor fluorescent probes based on symmetrical gold nanostars},
year = {2023},
journal = {Nano Research},
volume = {16},
number = {4},
pages = {5391-5400},
keywords = {nucleic acid multicolor fluorescent probe, symmetric gold nanostars, T-2 toxin, P53 mediated apoptosis pathway, living cells imaging},
url = {https://www.sciopen.com/article/10.1007/s12274-022-5069-9},
doi = {10.1007/s12274-022-5069-9},
abstract = {T-2 toxin, one of the most dangerous natural pollutants, induces apoptosis through multiple pathways. Amongst, P53 mediated apoptosis pathway, an important collection of molecules, plays a key role in cell vital activity. Real-time monitoring of upstream and downstream activation relationships of P53 mRNA, Bax mRNA, and cytochrome c (Cyt c) in signaling pathways is of great significance for understanding the apoptotic machinery in human physiology. In this work, a novel nucleic acid multicolor fluorescent probe, based on silica-coated symmetric gold nanostars (S-AuNSs@SiO2), was developed for highly sensitive in situ real-time imaging of P53 mRNA, Bax mRNA, and Cyt c during T-2 toxin-induced apoptosis. The nucleic acid chains modified with carboxyl groups were modified on the surface of S-AuNSs@SiO2 by amide reaction. The complementary chains of targeted mRNA and the aptamer of targeted Cyt c were modified with different fluorophores, respectively, and successfully hybridized on S-AuNSs@SiO2 surface. When targets were present, the fluorescent chains bound to the targets and detached from the material, resulting in the quenched fluorescence being revived. The probes based on S-AuNSs showed excellent performance is partly ascribed to the presence of 20 symmetric “hot spots”. Notably, the amide-bonded probe exhibited excellent anti-interference capability against biological agents (nucleases and biothiols). During the real-time fluorescence imaging of T-2 toxin-induced apoptosis, the corresponding fluorescence signals of P53 mRNA, Bax mRNA, and Cyt c were observed sequentially. Therefore, S-AuNSs@SiO2 probe not only provides a novel tool for real-time monitoring of apoptosis pathways cascade but also has considerable potential in disease diagnosis and pharmaceutical medical.}
}