@article{Lv2023, 
author = {Yu Lv and Mingshi Deng and Xuefeng Wang and Xinghua Gao and Lingyan Feng and Wei Chen and Chung-Chiun Liu and Xinxin Li and Yuan Zhang},
title = {In-situ formation of “electron conductive wires” threaded ZIF-8 membrane for multiplexed immunoassay of human interleukins},
year = {2023},
journal = {Nano Research},
volume = {16},
number = {2},
pages = {2866-2874},
keywords = {zeolitic imidazolate framework (ZIF), integrated nanostructure, microsensor, biomarker detection, interleukins},
url = {https://www.sciopen.com/article/10.1007/s12274-022-5030-y},
doi = {10.1007/s12274-022-5030-y},
abstract = {To address the challenge of highly sensitive and accurate detection of biomarkers in complex environments, a rational engineering strategy for designing electrochemical immunosensing platform is proposed. Herein, we develop a microsensor chip through the combination of multiplexed electrodes and microfluidic channels for the parallel detection of human interleukins (IL-6 and IL-8). For the construction of an efficient sensing interface, the conductive silver nanowires (Ag NWs) wrapped with zeolitic imidazolate framework (ZIF-8) thin film (denoted as ZIF-8@Ag NWs) are prepared, and then employed for the multi-functionalization of electrodes. The immunodetection of ILs is based on the direct signal transduction ability of Ag NWs and specific interaction of periodically arranged Zn2+ ions in ZIF-8 films with biomolecules, which offer the high assay sensitivity and good specificity. The immunosensor chip achieves a wide detection range from pg/mL to ng/mL and possesses the ability to resist non-specific proteins adsorption in biological complex media. Further clinical serum samples assay verifies that the combination of IL-6 and IL-8 levels yields high diagnostic accuracy. Principal component analysis (PCA) reveals that 18 patient samples could be fully separated from healthy control samples. The low-cost, easily fabricated electrochemical immunosensing platform provides a rapid serum test for diagnosis and personalized therapy of inflammatory diseases, and can also be generalized to other immunoreaction-based biomarkers detections.}
}