@article{Zhang2022, 
author = {Fuying Zhang and Donghao Hu and Xianlong Su and Zhengdao Hong and Wei Feng and Ming Xu and Fuyou Li},
title = {Two birds with one stone: Amine-functionalized MSNs@Eu(OH)CO3 nanoprobe for efficient dissolution-enhanced afterglow bioassay},
year = {2022},
journal = {Nano Research},
volume = {15},
number = {9},
pages = {8360-8366},
keywords = {bioassay, photochemical afterglow, mesoporous SiO2 nanoparticles (MSNs), nanocomposites, dissolution-enhanced},
url = {https://www.sciopen.com/article/10.1007/s12274-022-4464-6},
doi = {10.1007/s12274-022-4464-6},
abstract = {The efficient detection of tumor markers is crucial to early cancer diagnosis and monitoring. Here, we propose a unique “two birds with one stone” dissolution-enhanced afterglow bioassay based on amino-functionalized mesoporous SiO2 nanoparticles (MSNs-NH2) encapsulated Eu(OH)CO3 nanoprobes. These nanoprobes (i) stably conjugate to the antibody and (ii) are highly soluble in the acidic enhancer solution, and provide a high Eu3+ concentration. The released Eu3+ ions further activate the photochemical afterglow system to determine the alpha-fetoprotein (AFP) levels in clinical serum samples. Without the use of a real-time excitation source, the interference of autofluorescence and scattering from blood can be avoided. The demonstrated limit of detection (LOD) for AFP was as low as 0.12 ng·mL−1. This covalently connected dissolution-enhanced luminescent bioassay (DELBA) and novel afterglow system shows tremendous potential for ultrasensitive and rapid clinical diagnosis.}
}