@article{Tang2021, 
author = {Miaomiao Tang and Lu Zhang and Bin Song and Xiaoyuan Ji and Chenyu Wang and Houyu Wang and Hua Xu and Yuanyuan Su and Yao He},
title = {Long-term fundus fluorescence angiography and real-time diagnosis of retinal diseases in non-human primate-animal models},
year = {2021},
journal = {Nano Research},
volume = {14},
number = {11},
pages = {3840-3847},
keywords = {fluorescent silicon nanoparticles, long-term, real-time, bioimaging, retinal diseases},
url = {https://www.sciopen.com/article/10.1007/s12274-021-3302-6},
doi = {10.1007/s12274-021-3302-6},
abstract = {Fluorescein angiography (FA) is a standard imaging modality for evaluating vascular abnormalities in retina-related diseases, which is recognized as the major cause of vision loss. Long-term and real-time fundus angiography is of great importance in preclinical research, nevertheless remaining big challenges up to present. In this study, we demonstrate that long-term fluorescence imaging of retinal vessels is enabled through a kind of fluorescent nanoagents, which is made of small-sized (hydrodynamic diameter: ~ 3 nm) silicon nanoparticles (SiNPs) featuring strong fluorescence, robust photostability, lengthened blood residency and negligible toxicity. In particular, the presented SiNPs-based nanoagents are capable of imaging retinal capillaries in ~ 10 min, which is around 10-fold longer than that (~ 1 min) of fluorescein sodium (FS, known as the most widely used contrast agents for FA in clinic). Taking cynomolgus macaques as non-human primate-animal model, we further demonstrate the feasibility of real-time diagnosis of retinal diseases (e.g., age-related macular degeneration (AMD)) through dynamic monitoring of vascular dysfunction.}
}