@article{Xia2022, 
author = {Ming Xia and Md Rejaul Hoq and Pengwei Huang and Wen Jiang and Xi Jiang and Ming Tan},
title = {Bioengineered pseudovirus nanoparticles displaying the HA1 antigens of influenza viruses for enhanced immunogenicity},
year = {2022},
journal = {Nano Research},
volume = {15},
number = {5},
pages = {4181-4190},
keywords = {S60 nanoparticle, pseudovirus nanoparticle, influenza virus, influenza vaccine, hemagglutinin, norovirus},
url = {https://www.sciopen.com/article/10.1007/s12274-021-4011-x},
doi = {10.1007/s12274-021-4011-x},
abstract = {Even with implementation of current influenza vaccines, influenza still claims up to 500,000 lives worldwide annually, indicating a need for a better vaccine strategy. We have developed a technology to generate unique S60-HA1 pseudovirus nanoparticles (PVNPs) that display the receptor-binding HA1 domains of influenza viruses. Each self-assembled S60-HA1 PVNP consists of a T = 1 icosahedral S60 nanoparticle that resembles the inner shell of norovirus capsid and 60 surface-displayed HA1 antigens that are excellent vaccine targets. Soluble S60-HA1 PVNPs presenting HA1 antigens of H7N9 influenza virus subtypes have been produced efficiently in large amount. Their three-dimensional (3D) structures have been solved by cryogenic electron microscopy. The PVNP-displayed HA1 antigens react with HA-specific antibody, and retain authentic sialic acid binding specificity and hemagglutinate human erythrocytes. The PVNPs are highly immunogenic, eliciting high titers of HA1-specific antibodies in mice and the mouse sera strongly inhibited hemagglutinations of homologous and heterologous influenza virus HA proteins. Therefore, the S60-HA1 PVNPs may provide useful reagents to study influenza viruses and offer a potential new vaccine tactic to fight the deadly influenza disease.}
}