@article{Vitry2016, 
author = {Pauline Vitry and Rolando Rebois and Eric Bourillot and Ariane Deniset-Besseau and Marie-Joelle Virolle and Eric Lesniewska and Alexandre Dazzi},
title = {Combining infrared and mode synthesizing atomic force microscopy: Application to the study of lipid vesicles inside Streptomyces bacteria},
year = {2016},
journal = {Nano Research},
volume = {9},
number = {6},
pages = {1674-1681},
keywords = {nanoscale subsurface imaging, atomic force microscopy, acoustic microscopy, infrared microscopy, biology},
url = {https://www.sciopen.com/article/10.1007/s12274-016-1061-6},
doi = {10.1007/s12274-016-1061-6},
abstract = {We propose a new analytical approach combining vibrational spectroscopy and acoustic tomography for the detection and characterization of vesicles inside Streptomyces bacteria. Using atomic force microscopy and infrared spectroscopy (AFM-IR), we detect the presence of triglyceride vesicles. Their sizes in depth are measured with high accuracy using mode synthesizing atomic force microscopy (MS-AFM). We conducted a comparative study of AFM-IR and MS-AFM, and highlighted the advantages of the coupling of these techniques in having a full characterization (chemical, topographical, and volumetric) of a biological sample. With these complementary techniques, a complete access to the vesicle size distribution has been achieved with an accuracy of less than 50 nm. A 3D reconstruction of bacteria showing the in-depth distribution of vesicles is given to underline the great potential of the acoustic method.}
}