@article{Yu2016, 
author = {Meihua Yu and Yuting Niu and Jun Zhang and Hongwei Zhang and Yannan Yang and Elena Taran and Siddharth Jambhrunkar and Wenyi Gu and Peter Thorn and Chengzhong Yu},
title = {Size-dependent gene delivery of amine-modified silica nanoparticles},
year = {2016},
journal = {Nano Research},
volume = {9},
number = {2},
pages = {291-305},
keywords = {silica nanoparticles, gene delivery, plasmid DNA, particle sizes, cellular uptake},
url = {https://www.sciopen.com/article/10.1007/s12274-015-0909-5},
doi = {10.1007/s12274-015-0909-5},
abstract = {Silica-based nanoparticles are promising carriers for gene delivery applications. To gain insights into the effect of particle size on gene transfection efficiency, amine-modified monodisperse Stöber spheres (NH2-SS) with diameters of 125, 230, 330, 440, and 570 nm were synthesized. The in vitro transfection efficiencies of NH2-SS for delivering plasmid DNA encoding green fluorescent protein (GFP) (pcDNA3-EGFP, abbreviated as pcDNA, 6.1 kbp) were studied in HEK293T cells. NH2-SS with a diameter of 330 nm (NH2-SS330) showed the highest GFP transfection level compared to NH2-SS particles with other sizes. The transfection efficiency was found as a compromise between the binding capacity and cellular uptake performance of NH2-SS330 and pcDNA conjugates. NH2-SS330 also demonstrated the highest transfection efficiency for plasmid DNA (pDNA) with a bigger size of 8.9 kbp. To our knowledge, this study is the first to demonstrate the significance of particle size for gene transfection efficiency in silica-based gene delivery systems. Our findings are crucial to the rational design of synthetic vectors for gene therapy.}
}