@article{Liu2018, 
author = {Yan Liu and Datao Tu and Wei Zheng and Lianyu Lu and Wenwu You and Shanyong Zhou and Ping Huang and Renfu Li and Xueyuan Chen},
title = {A strategy for accurate detection of glucose in human serum and whole blood based on an upconversion nanoparticles-polydopamine nanosystem},
year = {2018},
journal = {Nano Research},
volume = {11},
number = {6},
pages = {3164-3174},
keywords = {polydopamine, upconversion nanoparticles, Förster resonance energy transfer, blood glucose, bioassay},
url = {https://www.sciopen.com/article/10.1007/s12274-017-1721-1},
doi = {10.1007/s12274-017-1721-1},
abstract = {The accurate detection of blood glucose is of critical importance in the diagnosis and management of diabetes and its complications. Herein, we report a novel strategy based on an upconversion nanoparticles-polydopamine (UCNPs-PDA) nanosystem for the accurate detection of glucose in human serum and whole blood through a simple blending of test samples with ligand-free UCNPs, dopamine, and glucose oxidase (GOx). Owing to the high affinity of lanthanide ions exposed on the surface of ligand-free UCNPs, dopamine monomers could spontaneously attach to the UCNPs and further polymerize to form a PDA shell, resulting in a remarkable upconversion luminescence (UCL) quenching (97.4%) of UCNPs under 980-nm excitation. Such UCL quenching can be effectively inhibited by H2O2 produced from the GOx/glucose enzymatic reaction, thus enabling the detection of H2O2 or glucose based on the UCL quenching/inhibition bioassay. Owing to the highly sensitive UCL response and background-free interference of the UCNPs-PDA nanosystem, we achieved a sensitive, selective, and high-throughput bioassay for glucose in human serum and whole blood, thereby revealing the great potential of the UCNPs-PDA nanosystem for the accurate detection of blood glucose or other H2O2-generated biomolecules in clinical bioassays.}
}