@article{Zhang2016, 
author = {Linlin Zhang and Yang Liu and Gan Liu and Duo Xu and Sheng Liang and Xinyuan Zhu and Yunfeng Lu and Hui Wang},
title = {Prolonging the plasma circulation of proteins by nano-encapsulation with phosphorylcholine-based polymer},
year = {2016},
journal = {Nano Research},
volume = {9},
number = {8},
pages = {2424-2432},
keywords = {protein therapy, phosphorylcholine-based polymer, nano-encapsulation, function protein delivery, long-circulation},
url = {https://www.sciopen.com/article/10.1007/s12274-016-1128-4},
doi = {10.1007/s12274-016-1128-4},
abstract = {Short in vivo circulation is a major hindrance to the widespread adoption of protein therapeutics. Protein nanocapsules generated by encapsulating proteins with a thin layer of phosphorylcholine-based polymer via a two-step encapsulation process exhibited significantly prolonged plasma half-life. Furthermore, by constructing nanocapsules with similar sizes but different surface charges and chemistry, we demonstrated a generic strategy for prolonging the plasma half-life of therapeutic proteins. In an in vitro experiment, four types of bovine serum albumin (BSA) nanocapsules were incubated with fetal bovine serum (FBS) in phosphate buffer saline (PBS); the cell uptake by HeLa cells was monitored to systematically evaluate the characteristics of the surface chemistry during circulation. Single positron emission tomography–computed tomography (SPECT) was employed to allow real-time observation of the BSA nanoparticle distribution in vivo, as well as quantification of the plasma concentration after intravenous administration. This study offers a practical method for translating a broad range of proteins for clinical use.}
}