@article{Luo2018, 
author = {Xiao Luo and Weiyu Zhao and Bin Li and Xinfu Zhang and Chengxiang Zhang and Anna Bratasz and Binbin Deng and David W. McComb and Yizhou Dong},
title = {Co-delivery of mRNA and SPIONs through amino-ester nanomaterials},
year = {2018},
journal = {Nano Research},
volume = {11},
number = {10},
pages = {5596-5603},
keywords = {magnetic resonance imaging (MRI), amino-ester nanomaterials, lipid-like nanoparticles (LLNs), dual-functional, superparamagnetic iron oxide nanoparticles (SPIONs), mRNA delivery},
url = {https://www.sciopen.com/article/10.1007/s12274-018-2082-0},
doi = {10.1007/s12274-018-2082-0},
abstract = {Nanoparticles have been widely explored for combined therapeutic and diagnostic applications. For example, lipid-based nanoparticles have been used to encapsulate multiple types of agents and achieve multi-functions. Herein, we enabled a co-delivery of mRNA molecules and superparamagnetic iron oxide nanoparticles (SPIONs) by using an amino-ester lipid-like nanomaterial. An orthogonal experimental design was used to identify the optimal formulation. The optimal formulation, MPA-Ab-8 LLNs, not only showed high encapsulation of both mRNA and SPIONs, but also increased the r2 relaxivity of SPIONs by more than 1.5-fold in vitro. MPA-Ab-8 LLNs effectively delivered mRNA and SPIONs into cells, and consequently induced high protein expression as well as strong MRI contrast. Consistent herewith, we observed both mRNA-mediated protein expression and an evident negative contrast enhancement of MRI signal in mice. In conclusion, amino-ester nanomaterials demonstrate great potential as delivery vehicles for theranostic applications.}
}