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Review | Publishing Language: Chinese | Open Access

Research Progress on Specific Recognition and Interaction Mechanism between Antibody and Antigen in Immunoassay for Heavy Metal Residues

Junjun HUANG , Mengxin ZHOU, Xiaodan LÜ, Zhili XIAO ( )
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China
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Abstract

Heavy metals are highly toxic and can contaminate a wide range of foods. Their pollution and residues can bring about serious harm to food safety. Therefore, it is critical to strengthen efforts to detect heavy metal residues in food products. Immunoassay is promising for the rapid and efficient detection of heavy metals. Heavy metal antibodies are at the heart of immunoassays for the detection of heavy metals. Heavy metal antigens consist of two parts: heavy metal ions and chelating agents. Due to the structural similarity among metal ions and the universality of chelating agents, the resulting antibodies often show inadequate specificity, although antibodies with good specificity have been reported in some literatures. Therefore, it is significant to explore the specific recognition and interaction mechanism between heavy metal antibodies and antigens. In this article, we elaborate three research methods for understanding the recognition mechanism between heavy metal antibodies and antigens and recent progress in this area, and analyze the critical factors for the specific recognition and interaction. We hope that this review will provide a reference for enhancing the performance of heavy metal antibodies, and lay the foundation for establishing efficient, sensitive and specific methods for the detection of heavy metal residues in foods.

Keywords

heavy metalsantibodyspecific recognitioninteraction mechanism
CLC number: TS201.6 Document code: A Article ID: 1002-6630(2025)05-0301-09

References

[2]

SCUTARAȘU E C, TRINCĂ L C. Heavy metals in foods and beverages: global situation, health risks and reduction methods[J]. Foods, 2023, 12(18): 3340. DOI:10.3390/foods12183340.
Crossref Google Scholar
[6]

CHEN X H, ZHAO J H, ZHOU L. Knowledge protects against pollution: the health effects of the cadmium rice event in China[J]. World Development, 2024, 175: 106470. DOI:10.1016/j.worlddev.2023.106470.
Crossref Google Scholar
[7]

VICENTE-MARTÍNEZ Y, MUÑOZ-SANDOVAL M J, HERNANDEZ-CORDOBA M, et al. Determination of Hg(Ⅱ) and methylmercury by electrothermal atomic absorption spectrometry after dispersive solid-phase microextraction with a graphene oxide magnetic material[J]. Molecules, 2022, 28(1): 14. DOI:10.3390/molecules28010014.
Crossref Google Scholar
[8]

CHATTERJEE A, DAS R, ABRAHAM J. Bioleaching of heavy metals from spent batteries using Aspergillus nomius JAMK1[J]. International Journal of Environmental Science and Technology, 2020, 17(1): 49-66. DOI:10.1007/s13762-019-02255-0.
Crossref Google Scholar
[9]

MANOUSI N, ZACHARIADIS G A. Development and validation of an ICP-AES method for the determination of toxic and nutrient metals in candies: application for the analysis of different samples from the Greek market[J]. Applied Sciences, 2021, 11(22): 10599. DOI:10.3390/app112210599.
Crossref Google Scholar
[10]

LI K J, YANG H Y, YUAN X, et al. Recent developments of heavy metals detection in traditional Chinese medicine by atomic spectrometry[J]. Microchemical Journal, 2021, 160: 105726. DOI:10.1016/j.microc.2020.105726.
Crossref Google Scholar
[12]

PANDEY S C, KUMAR A, SAHU S K. Single step green synthesis of carbon dots from Murraya koenigii leaves; a unique turn-off fluorescent contrivance for selective sensing of Cd(Ⅱ) ion[J]. Journal of Photochemistry and Photobiology A: Chemistry, 2020, 400: 112620. DOI:10.1016/j.jphotochem.2020.112620.
Crossref Google Scholar
[13]

HELEN R R, RAHALE C S, GIRIJA S, et al. Electrochemical detection of mercury ions using glassy carbon electrode modified with silver sulphide nanoparticles[J]. Physica Scripta, 2024, 99(10): 105401. DOI:10.1088/1402-4896/ad72ac.
Crossref Google Scholar
[15]

XING Y M, WU X L, LIU L Q, et al. Development of a fluorescent immunoassay strip for the rapid quantitative detection of cadmium in rice[J]. Food and Agricultural Immunology, 2020, 31(1): 501-512. DOI:10.1080/09540105.2020.1741518.
Crossref Google Scholar
[20]

XU W, XIE P, FAN L Y, et al. Synthesis and characteristics of a novel artificial hapten using the copper mercaptide of penicillenic acid from penicillin G for immunoassay of heavy metal ions[J]. Science China Life Sciences, 2011, 54(9): 813-821. DOI:10.1007/s11427-011-4220-8.
Crossref Google Scholar
[21]

WESTHOFF C M, LOPEZ O, GOEBEL P, et al. Unusual amino acid usage in the variable regions of mercury-binding antibodies[J]. Proteins: Structure, Function, and Bioinformatics, 1999, 37(3): 429-440. DOI:10.1002/(SICI)1097-0134(19991115)37:3<429:AID-PROT10>3.0.CO;2-P.
Crossref Google Scholar
[33]

FU X S, LI C F, JI Q G, et al. Establishment of indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for copper ion (Cu2+) in raw meat products[J]. Journal of Food Protection, 2024, 87(5): 100262. DOI:10.1016/j.jfp.2024.100262.
Crossref Google Scholar
[36]

FU X S, CHEN E J, MA B, et al. Establishment of an indirect competitive enzyme-linked immunosorbent method for the detection of heavy metal cadmium in food packaging materials[J]. Foods, 2021, 10(2): 413. DOI:10.3390/foods10020413.
Crossref Google Scholar
[37]

XIAO Z L, TIAN W, XU Z L, et al. Development of a chemiluminescent enzyme immunoassay to detect cadmium in cereal samples[J]. Food and Agricultural Immunology, 2018, 29(1): 1184-1196. DOI:10.1080/09540105.2018.1527295.
Crossref Google Scholar
[38]

XU N F, ZHU Q J, ZHU J X, et al. Novel latex microsphere immunochromatographic assay for rapid detection of cadmium ion in Asparagus[J]. Foods, 2021, 11(1): 78. DOI:10.3390/foods11010078.
Crossref Google Scholar
[39]

SARADA N C, THAMARAISELVI K, VIJAYALAKSHMI M A. Anti Zn antibodies: cross reactivity and competitive binding with heavy metals[J]. Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences, 2008, 861(2): 236-239. DOI:10.1016/j.jchromb.2007.08.035.
Crossref Google Scholar
[40]

ZHU X X, HU B S, LOU Y, et al. Characterization of monoclonal antibodies for lead-chelate complexes: applications in antibody-based assays[J]. Journal of Agricultural and Food Chemistry, 2007, 55(13): 4993-4998. DOI:10.1021/jf070787d.
Crossref Google Scholar
[41]

JONES R M, YU H N, DELEHANTY J B, et al. Monoclonal antibodies that recognize minimal differences in the three-dimensional structures of metal-chelate complexes[J]. Bioconjugate Chemistry, 2002, 13(3): 408-415. DOI:10.1021/bc0155418.
Crossref Google Scholar
[42]

KHOSRAVIANI M, BLAKE R C, PAVLOV A R, et al. Binding properties of a monoclonal antibody directed toward lead-chelate complexes[J]. Bioconjugate Chemistry, 2000, 11(2): 267-277. DOI:10.1021/bc9901548.
Crossref Google Scholar
[43]

BLAKE D A, CHAKRABARTI P, KHOSRAVIANI M, et al. Metal binding properties of a monoclonal antibody directed toward metal-chelate complexes[J]. Journal of Biological Chemistry, 1996, 271(44): 27677-27685. DOI:10.1074/jbc.271.44.27677.
Crossref Google Scholar
[44]

BODEN V, COLIN C, BARBET J, et al. Preliminary study of the metal binding site of an anti-DTPA-indium antibody by equilibrium binding immunoassays and immobilized metal ion affinity chromatography[J]. Bioconjugate Chemistry, 1995, 6(4): 373-379. DOI:10.1021/bc00034a006.
Crossref Google Scholar
[45]

DELEHANTY J B, JONES R M, BISHOP T C, et al. Identification of important residues in metal-chelate recognition by monoclonal antibodies[J]. Biochemistry, 2003, 42(48): 14173-14183. DOI:10.1021/bi034839d.
Crossref Google Scholar
[46]

LOVE R A, VILLAFRANCA J E, AUST R M, et al. How the anti-(metal chelate) antibody CHA255 is specific for the metal ion of its antigen: X-ray structures for two Fab’/hapten complexes with different metals in the chelate[J]. Biochemistry, 1993, 32(41): 10950-10959. DOI:10.1021/bi00092a004.
Crossref Google Scholar
[48]

BEATTY J D, BEATTY B G, VLAHOS W G. Measurement of monoclonal antibody affinity by non-competitive enzyme immunoassay[J]. Journal of Immunological Methods, 1987, 100(1/2): 173-179. DOI:10.1016/0022-1759(87)90187-6.
Crossref Google Scholar
[50]

LOOMANS E E M G, ROELEN A J M, VAN DAMME H S, et al. Assessment of the functional affinity constant of monoclonal antibodies using an improved enzyme-linked immunosorbent assay[J]. Journal of Immunological Methods, 1995, 184(2): 207-217. DOI:10.1016/0022-1759(95)00089-S.
Crossref Google Scholar
[53]

BLAKE R C, PAVLOV A R, BLAKE D A. Automated kinetic exclusion assays to quantify protein binding interactions in homogeneous solution[J]. Analytical Biochemistry, 1999, 272(2): 123-134. DOI:10.1006/abio.1999.4176.
Crossref Google Scholar
[54]

HARMS B D, KEARNS J D, SU S V, et al. Optimizing properties of antireceptor antibodies using kinetic computational models and experiments[J]. Methods in Enzymology, 2012, 502: 67-87. DOI:10.1016/B978-0-12-416039-2.00004-5.
Crossref Google Scholar
[55]

DARLING R J, BRAULT P A. Kinetic exclusion assay technology: characterization of molecular interactions[J]. Assay and Drug Development Technologies, 2004, 2(6): 647-657. DOI:10.1089/adt.2004.2.647.
Crossref Google Scholar
[60]

WU J T, LV J, ZHAO L, et al. Exploring the role of microbial proteins in controlling environmental pollutants based on molecular simulation[J]. Science of the Total Environment, 2023, 905: 167028. DOI:10.1016/j.scitotenv.2023.167028.
Crossref Google Scholar
[61]

DÜREN T, BAE Y S, SNURR R Q. Using molecular simulation to characterise metal-organic frameworks for adsorption applications[J]. Chemical Society Reviews, 2009, 38(5): 1237-1247. DOI:10.1039/b803498m.
Crossref Google Scholar
[64]

AKBAR S, MOZUMDER S, SENGUPTA J. Retrospect and prospect of single particle cryo-electron microscopy: the class of integral membrane proteins as an example[J]. Journal of Chemical Information and Modeling, 2020, 60(5): 2448-2457. DOI:10.1021/acs.jcim.9b01015.
Crossref Google Scholar
[68]

STURA E A, FIESER G G, WILSON I A. Crystallization of antibodies and antibody-antigen complexes[J]. ImmunoMethods, 1993, 3(3): 164-179. DOI:10.1006/immu.1993.1051.
Crossref Google Scholar
[69]

HE K, DU X J, SHENG W, et al. Crystal structure of the fab fragment of an anti-ofloxacin antibody and exploration of its specific binding[J]. Journal of Agricultural and Food Chemistry, 2016, 64(12): 2627-2634. DOI:10.1021/acs.jafc.5b05882.
Crossref Google Scholar
[70]

PARK S Y, LEE W R, LEE S C, et al. Crystal structure of single-domain VL of an anti-DNA binding antibody 3D8 scFv and its active site revealed by complex structures of a small molecule and metals[J]. Proteins, 2008, 71(4): 2091-2096. DOI:10.1002/prot.22011.
Crossref Google Scholar
[72]

BLAKE D A, PAVLOV A R, YU H N, et al. Antibodies and antibodybased assays for hexavalent uranium[J]. Analytica Chimica Acta, 2001, 444(1): 3-11. DOI:10.1016/S0003-2670(01)01151-5.
Crossref Google Scholar
[73]

WANG Y L, ZHANG C Z, LIU F Q. Antibody developments for metal ions and their applications[J]. Food and Agricultural Immunology, 2020, 31(1): 1079-1103. DOI:10.1080/09540105.2020.1828293.
Crossref Google Scholar
[74]

BLAKE R C, PAVLOV A R, KHOSRAVIANI M, et al. Novel monoclonal antibodies with specificity for chelated uranium(Ⅵ): isolation and binding properties[J]. Bioconjugate Chemistry, 2004, 15(5): 1125-1136. DOI:10.1021/bc049889p.
Crossref Google Scholar
[77]

WANG Y Z, YANG H, PSCHENITZA M, et al. Highly sensitive and specific determination of mercury(Ⅱ) ion in water, food and cosmetic samples with an ELISA based on a novel monoclonal antibody[J]. Analytical and Bioanalytical Chemistry, 2012, 403(9): 2519-2528. DOI:10.1007/s00216-012-6052-1.
Crossref Google Scholar
[78]

WYLIE D E, LU D, CARLSON L D, et al. Monoclonal antibodies specific for mercuric ions[J]. Proceedings of the National Academy of Sciences of the United States of America, 1992, 89(9): 4104-4108. DOI:10.1073/pnas.89.9.4104.
Crossref Google Scholar
[79]

WANG F Y, LI N, ZHANG Y S, et al. Preparation and directed evolution of anti-ciprofloxacin ScFv for immunoassay in animal-derived food[J]. Foods, 2021, 10(8): 1933. DOI:10.3390/foods10081933.
Crossref Google Scholar
[81]

LING S M, DONG M K, XU Y, et al. Development of sensitive and portable immunosensors based on signal amplification probes for monitoring the mercury(Ⅱ) ions[J]. Biosensors and Bioelectronics, 2022, 217: 114676. DOI:10.1016/j.bios.2022.114676.
Crossref Google Scholar
[82]

HAN Z, PAN Y, CHEN S H, et al. Time-resolved FRET-based immunosensor for the ultrasensitive and rapid detection of Cd2+[J]. Journal of Agricultural and Food Chemistry, 2023, 71(29): 11195-11203. DOI:10.1021/acs.jafc.3c01535.
Crossref Google Scholar
[84]

TIAN W, WANG S X, LI X M, et al. An automatic and smart platform for rapid detection of cadmium and lead simultaneously in rice using triple-amplified chemiluminescence immunoassay[J]. Food Chemistry, 2024, 437: 137900. DOI:10.1016/j.foodchem.2023.137900.
Crossref Google Scholar
[87]

XU L, SUO X Y, ZHANG Q, et al. ELISA and chemiluminescent enzyme immunoassay for sensitive and specific determination of lead(Ⅱ) in water, food and feed samples[J]. Foods, 2020, 9(3): 305. DOI:10.3390/foods9030305.
Crossref Google Scholar
[90]

LV S, XU X X, SONG S S, et al. An immunochromatographic assay for the rapid and qualitative detection of mercury in rice[J]. Biosensors, 2022, 12(9): 694. DOI:10.3390/bios12090694.
Crossref Google Scholar
[91]

WANG X F, WANG Y N, WANG S Y, et al. Indirect competitive ELISA for the determination of total chromium content in food, feed and environmental samples[J]. Molecules, 2022, 27(5): 1585. DOI:10.3390/molecules27051585.
Crossref Google Scholar
Food Science
Volume 46 Issue 5,
March 2025
Pages 301-309
DOI: 10.7506/spkx1002-6630-20240715-139
Cite this article:
HUANG J, ZHOU M, LÜ X, et al. Research Progress on Specific Recognition and Interaction Mechanism between Antibody and Antigen in Immunoassay for Heavy Metal Residues. Food Science, 2025, 46(5): 301-309. https://doi.org/10.7506/spkx1002-6630-20240715-139

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Received: 15 July 2024
Published: 15 March 2025
© Beijing Academy of Food Sciences 2025.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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