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20 April 2021
Probing lysosomal activity in vivo
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Lysosomes are membrane-bound organelles for biomolecule degradation and recycling. They also serve as a nutrient sensing and signaling center to maintain cell and tissue homeostasis. Lysosomal properties alter in response to developmental or environmental cues, but these changes are hard to track in vivo. Employing C. elegans as a model system, we have developed assays to examine and quantify lysosome properties in vivo, including lysosome maturation, acidification and cleavage activity. These assays can be used to reveal alterations of lysosomal activity during C. elegans development and in stress conditions.
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Probing lysosomal activity in vivo
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Abstract
Lysosomes are membrane-bound organelles for biomolecule degradation and recycling. They also serve as a nutrient sensing and signaling center to maintain cell and tissue homeostasis. Lysosomal properties alter in response to developmental or environmental cues, but these changes are hard to track in vivo. Employing C. elegans as a model system, we have developed assays to examine and quantify lysosome properties in vivo, including lysosome maturation, acidification and cleavage activity. These assays can be used to reveal alterations of lysosomal activity during C. elegans development and in stress conditions.
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Acknowledgements
Some strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40OD010440). This work was supported by the National Natural Science Foundation of China (3163001, 91754203), the Ministry of Science and Technology (2016YFA0500203), and the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB19000000) to X. Wang. X. Li is supported by the National Natural Science Foundation of China (31671401).
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