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Research Article | Open Access

The Excretion of BRCAA Antibody-Conjugated Gold Nanoclusters from Kidneys of Treated Rats

Kunlu Liu1,#, Haixia Li1,#, Yajun Wu1, Chunlei Zhang2, Duanyun Si3, Quansheng Li3, Sa Zhang4, Jiye Yin1, Yuanyuan Liu1, Wensheng Qu1, Junhua Wu1, Yingge Zhang1, Ruibin Su1, Yuxia Wang1( )
State Key Laboratory of Toxicology and Medical Countermeasures, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China
Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China
Tianjin State Key Laboratory of Pharmacokinetics and Pharmacodynamics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China
National Center of Biomedical Analysis, Academy of Military Medical Sciences, Beijing 100850, China

#Authors equally contributed to the work.

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Abstract

The gold nanoclusters (Au-NCs) have emerged for delivery of various payloads such as small molecular drug and big molecular proteins due to their low immunogenicity, quick clearance and good biocompatibility. Even though they have shown great prospective in biomedical area, there are also some security problems which should be investigated further more. BRCAA1 mAb Au-NCs is a probe for gastric cancer diagnosis prepared by conjugating BRCAA1 monoclonal antibody with fluorescent Au-NCs. In this research, the renal excretion and effect on glomerulus filtration of BRCAA1 mAb AuNCs in rats were investigated. The result indicated that BRCAA1 mAb Au-NCs could be eliminated through the glomerulus filtration as early as 2 hr after the administration following the excretion of other proteins in the plasma such as immunoglobulin. Electron microscope image and pathological analysis of kidney showed that BRCAA1 mAb Au-NCs induced some structural injury such as capillaries endotheliocyte damage, pycnosis, karyorrhexis and hyalomitome acidophilia enhancement. Biochemical indexes including ALT, AST, BUN, TBIL, CK and CREA are abnormal in the period of 2 to 48 hr after the administration. The results of Western blotting and immunohistochemisty show the downregulation of three key proteins (ZO-1, Nephrin and Podocin) indicating the influence of BRCAA1 mAb Au-NCs on the foot processes of glomerular epithelial cells and shallow intercellular junctions or slit diaphragms.

Keywords

Gold nanoclustersBRCAA1 antibodyRenal excretionGlomerular filtration

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Nano Biomedicine and Engineering
Volume 9 Issue 1,
March 2017
Pages 36-48
DOI: 10.5101/nbe.v9i1.p36-48
Cite this article:
Liu K, Li H, Wu Y, et al. The Excretion of BRCAA Antibody-Conjugated Gold Nanoclusters from Kidneys of Treated Rats. Nano Biomedicine and Engineering, 2017, 9(1): 36-48. https://doi.org/10.5101/nbe.v9i1.p36-48

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Received: 15 March 2017
Accepted: 17 March 2017
Published: 24 March 2017
© 2017 Kunlu Liu, Haixia Li, Yajun Wu, Chunlei Zhang, Duanyun Si, Quansheng Li, Sa Zhang, Jiye Yin, Yuanyuan Liu, Wensheng Qu, Junhua Wu, Yingge Zhang, Ruibin Su, and Yuxia Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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