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Nano Biomedicine and Engineering 2019, 11(1): 35-43 https://doi.org/10.5101/nbe.v11i1.p35-43
Research Article | Open Access | Issue | Published: 28 February 2019

Zinc Oxide Nanoparticles Induces Apoptosis in Human Breast Cancer Cells via Caspase-8 and P53 Pathway

Show Author's Information Kadhem Haitham Ali1,  Sumayah Abdulhussien Ibraheem1,  Majid Sakhi Jabir2( ),  Kadhim Afraa Ali3,  Zainab Jihad Taqi2,  Florin Mihailescu Dan1
Department of Animal Physiology and Biophysics, Faculty of Biology, Bucharest, Romania
Department of Applied Science, University of Technology, Baghdad, Iraq
Department of Biology, College of Science, University of Al-Mustansiriyah, Baghdad, Iraq
Keywords:
Cytotoxicity, Zinc oxide nanoparticles, Genotoxicity, P53, MCF-7, Caspase-8
Cite this article:
Ali KH, Ibraheem SA, Jabir MS, et al. Zinc Oxide Nanoparticles Induces Apoptosis in Human Breast Cancer Cells via Caspase-8 and P53 Pathway. Nano Biomedicine and Engineering, 2019, 11(1): 35-43. https://doi.org/10.5101/nbe.v11i1.p35-43
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Abstract Full text About this article

Nanoparticles are a special institution of substances with precise capabilities and significant applications in many biomedical fields. In the present work zinc oxide nanoparticles were prepared through sol-gel approach. The synthesised nanoparticles were identified through the usage of X-ray powder diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). In-vitro anticancer activity of zinc oxide nanoparticles towards MCF-7 cell lines using numerous parameters was investigated. Zinc oxide nanoparticles were determined to exert cell growth arrest against MCF-7 cell lines. The anti-proliferative efficiency of ZnO nanoparticles was due to cell dying and inducing apoptosis that were confirmed by the usage of acridine orange/ethidium bromide dual staining, DAPI staining and genotoxicity assay. Reverse transcription polymerase chain reaction (RT- PCR) analysis achieved to identify the gene expression of Caspase-8, Caspase-9, and P53. The results suggested that ZnO nanoparticles might find a wide use in clinical applications and provide new drug recompense for chemotherapy drugs.


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About this article

Zinc Oxide Nanoparticles Induces Apoptosis in Human Breast Cancer Cells via Caspase-8 and P53 Pathway

Show Author's information Kadhem Haitham Ali1, Sumayah Abdulhussien Ibraheem1, Majid Sakhi Jabir2( ), Kadhim Afraa Ali3, Zainab Jihad Taqi2, Florin Mihailescu Dan1
Department of Animal Physiology and Biophysics, Faculty of Biology, Bucharest, Romania
Department of Applied Science, University of Technology, Baghdad, Iraq
Department of Biology, College of Science, University of Al-Mustansiriyah, Baghdad, Iraq

Abstract

Nanoparticles are a special institution of substances with precise capabilities and significant applications in many biomedical fields. In the present work zinc oxide nanoparticles were prepared through sol-gel approach. The synthesised nanoparticles were identified through the usage of X-ray powder diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). In-vitro anticancer activity of zinc oxide nanoparticles towards MCF-7 cell lines using numerous parameters was investigated. Zinc oxide nanoparticles were determined to exert cell growth arrest against MCF-7 cell lines. The anti-proliferative efficiency of ZnO nanoparticles was due to cell dying and inducing apoptosis that were confirmed by the usage of acridine orange/ethidium bromide dual staining, DAPI staining and genotoxicity assay. Reverse transcription polymerase chain reaction (RT- PCR) analysis achieved to identify the gene expression of Caspase-8, Caspase-9, and P53. The results suggested that ZnO nanoparticles might find a wide use in clinical applications and provide new drug recompense for chemotherapy drugs.

Keywords: Cytotoxicity, Zinc oxide nanoparticles, Genotoxicity, P53, MCF-7, Caspase-8

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Publication history
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Publication history

Received: 18 May 2018
Accepted: 19 November 2018
Published: 28 February 2019
Issue date: March 2019

Copyright

© Haitham Ali Kadhem, Sumayah Abdulhussien Ibraheem, Majid Sakhi Jabir, Afraa Ali Kadhim, Zainab Jihad Taqi, and Mihailescu Dan Florin.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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