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Publishing Language: Chinese

Effects of Wild Populations for Hybridization and Outdoor Population Exercise on the Parasitic Ability of Aphidius gifuensis and Enzymes Related to the Growth and Development

JiaoPing HE1, Meng HAN1, Ting ZHOU2, Gang GU2( ), YuFei LAI3, RongQuan LAI1( )
Institute of Biological Control, College of Plant Protection/State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002
Fujian Institute of Tobacco Science, China National Tobacco Corporation, Fuzhou 350003
College of Plant Protection, Shandong Agricultural University, Taian 271018, Shandong
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Abstract

【Objective】

Aphidius gifuensis is the dominant parasitic wasp of Myzus persicae, however there are some problems in the process of artificial or large-scale production and propagation of A. gifuensis, such as the body size becoming smaller and the parasitism decreasing. The purpose of this study is to analyze the effects of different regulation methods on the parasitism rate, body size and the content and activity of enzymes related to the growth and development of offspring A. gifuensis, so as to improve the population rejuvenation technology of the degraded A. gifuensis and ensure the scale and stability of artificial reproduction.

【Method】

Indoor selection of A. gifuensis, which showed significant population degradation after 10 generations, was conducted for wild population hybridization rejuvenation, indoor exercise rejuvenation, and outdoor exercise rejuvenation. The formation of stiff aphids was observed every 12 h. After the formation of stiff aphids (during the yellow brown period of stiff aphids), the number was counted and the parasitism rate of A. gifuensis was calculated. After the metamorphosis of the aphid, six representative female adults A. gifuensis that could reflect the overall characteristics of each treatment group were randomly selected and the metathoracic tibia length was measured under a stereomicroscope as an indicator of body size. The stiff aphids with different treatments were collected and placed separately. The standard curve was made by Coomassie brilliant blue G-250 method within 12 h after emergence, and the content and activity of growth and development related enzymes were determined by ultraviolet spectrophotometer colorimetry.

【Result】

Wild population hybridization rejuvenation and outdoor exercise rejuvenation had good regulatory effects on the parasitic ability of A. gifuensis (P<0.05), but indoor exercise rejuvenation had no significant regulatory effect on the parasitic ability of A. gifuensis (P>0.05). The hybridization between wild female A. gifuensis with the indoor degraded male one (1∶1) had the best rejuvenation effect on the parasitic ability. Compared with the control group, the parasitism rate and offspring body size were increased by 17.83% and 18.98%, respectively. The contents of carboxylesterase, phenoloxidase and trehalase in the offspring and the activity of phenoloxidase were significantly increased (P<0.05). After six generations of outdoor exercise, compared with the 0 generations, the parasitism rate and body size of offspring A. gifuensis were increased by 24.67% and 49.33%, respectively, and the contents of carboxylesterase, phenoloxidase, superoxide dismutase (SOD) and trehalase in the offspring were increased significantly (P<0.05), the activities of phenoloxidase and SOD were also significantly increased (P<0.05).

【Conclusion】

In production, the field hybridization rejuvenation and outdoor exercise rejuvenation of the degraded A. gifuensis population can improve the parasitic ability of A. gifuensis to M. persicae.

Keywords

Aphidius gifuensis Myzus persicae parasitic abilityrejuvenationenzymehybridization

References

[1]
AN T, ZHANG H Z, HAN Y H, CHEN H Y, ZHANG L S. Transcriptome analysis of diapause-associated genes of Aphidius gifuensis Ashmaed. Chinese Journal of Biological Control, 2017, 33(5): 604-611. (in Chinese)
Google Scholar
[2]
LAI R Q, YOU M S, ZHU C Z, GU G, LIN Z L, LIAO L L, LIN L T, ZHONG X J. Myzus persicae and aphid-transmitted viral disease control via variety intercropping in flue-cured tobacco. Crop Protection, 2017, 100: 157-162.
Crossref Google Scholar
[3]
LAI R Q, YOU M S, LOTZ L A P, VASSEUR L. Response of green peach aphids and other arthropods to garlic intercropped with tobacco. Agronomy Journal, 2011, 103(3): 856-863.
Crossref Google Scholar
[4]
YU Y C, YANG M F, SHANG S H, LIU J F, YU X F, DI X Y. Effects of herbivore induced tobacco resistance on feeding and detoxification enzymes of Spodoptera litura (Lepidoptera: Noctuidae). Journal of Nuclear Agricultural Sciences, 2022, 36(1): 236-243. (in Chinese)
Google Scholar
[5]
BAI J J, GU G, LAI R Q, ZHOU T, WU X T, CHEN D M, SHU J. Effects of juvenile hormone analogs on the growth and development, parasitism rate and activities of molting-related enzymes in Aphidius gifuensis (Hymenoptera: Aphidiidae). Acta Entomologica Sinica, 2020, 63(9): 1091-1100. (in Chinese)
Google Scholar
[6]
BAI J J, GU G, LAI R Q, ZHOU T, MI Y E, HAN M, ZHANG B. Effects of temperature and juvenile hormone analogs on the emergence time and rate of Aphidius gifuensis (Hymenoptera: Aphidiidae). Acta Tabacaria Sinica, 2022, 28(2): 131-137. (in Chinese)
Google Scholar
[7]
WANG Q N, LAI R Q, GU G, WU X T, BAI J J, MI Y E, SHU J, GAO G G. Parasitic effects of different generations of Aphidius gifuensis on Myzus persicae in Fujian flue-cured tobacco district. Chinese Journal of Biological Control, 2021, 37(5): 963-969. (in Chinese)
Google Scholar
[8]
XIE Y Q, ZHANG H Z, LI Y Y, KONG L, XIANG M, YANG H L, ZHANG L M, AI H M, ZHANG L S. Population degradation rule of Aphidius gifuensis (Hymenoptera: Aphidiidae). Chinese Journal of Biological Control, 2020, 36(2): 163-168. (in Chinese)
Google Scholar
[9]
MA F H, ZHOU T, LAI R Q, GU G, HAN M. Effects of tobacco varieties, seedling raising methods and sources of Myzus persicae (Hemiptera: Aphididae) on the parasitic ability of Aphidius gifuensis (Hymenoptera: Braconidae). Acta Entomologica Sinica, 2023, 66(1): 85-91. (in Chinese)
Google Scholar
[10]
DAI P L. Research of important hymenopterous parasitoid Scleroderma guani Xiao et Wu: Embryonic development, parasitizing behavior and population rejuvenation[D]. Beijing: China Agricultural University, 2005. (in Chinese)
[11]
LI H. Studies on rejuvenescence of Chouioia cunea Yang[D]. Taian: Shandong Agricultural University, 2011. (in Chinese)
[12]
DU Q X, CAI H L, LI J Y, WU S L, ZENG W A, ZHAI Z G, YANG Z X. Effects of field rejuvenation on biological characteristics of Arma chinensis and its progeny. Chinese Journal of Biological Control, 2022, 38(6): 1619-1624. (in Chinese)
Google Scholar
[13]
LAI R Q, HAN M, GU G, ZHOU T, ZHANG B, LAI Y F, YANG C. Effects of supplemental nutrition and trace elements on the parasitic ability of Aphidius gifuensis (Hymenoptera: Braconidae) and growth and development-related enzymes. Acta Entomologica Sinica, 2024, 67(2): 203-212. (in Chinese)
Google Scholar
[14]
REN N N, XIE M, YOU Y C, YOU M S. An overview on the study of insect carboxylesterases (COEs) and the COE-mediated resistance to insecticides. Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2014, 43(4): 337-344. (in Chinese)
Google Scholar
[15]
XU Y L, LI W C. Research progress in activation mechanisms of phenoloxidse in insects. Journal of Anhui Agricultural Sciences, 2010, 38(27): 14844-14846. (in Chinese)
Google Scholar
[16]
BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 1976, 72: 248-254.
Crossref Google Scholar
[17]
HE Y Q. Studies on Aphidius gifuensis-Myzus persicae-PVY interactions[D]. Chongqing: Southwest University, 2017. (in Chinese)
[18]
VAN ASPEREN K. A study of housefly esterases by means of a sensitive colorimetric method. Journal of Insect Physiology, 1962, 8(4): 401-416.
Crossref Google Scholar
[19]
CHANG J, ZHANG W, LI H P, DIAO Q Y. Study on susceptibility to pyrethroids and carboxylesterase activity in Myzus persicae on potato. Chinese Journal of Pesticide Science, 2016, 18(2): 201-206. (in Chinese)
Google Scholar
[20]
LIU S Z. The immunological function of insect phenoloxidase and its response to external interference factors including insecticides[D]. Taian: Shandong Agricultural University, 2008. (in Chinese)
[21]
BENJAMIN N D, MONTGOMERY M W. Polyphenol oxidase of Royal Ann cherries: Purification and characterization. Journal of Food Science, 1973, 38(5): 799-806.
Crossref Google Scholar
[22]
ZHANG J H, WANG W, LIN S H, LIU W, SU D L, LI G Y. Effect of cloves, Schisandra chinensis and chitosan compound preservative on storage quality and disease-resistant enzyme activity of blueberries. Journal of Chinese Institute of Food Science and Technology, 2017, 17(12): 139-148. (in Chinese)
Google Scholar
[23]
ZHANG H, WANG X R, KUANG S Z, WU J F, LÜ Z Z. Effect of sublethal concentration of insecticides and SINPV on the activities of SOD, CAT and POD in Spodoptera litura. Acta Entomologica Sinica, 2006, 49(5): 775-779. (in Chinese)
Google Scholar
[24]
BEAUCHAMP C, FRIDOVICH I. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 1971, 44(1): 276-287.
Crossref Google Scholar
[25]
MALECKA A, JARMUSZKIEWICZ W, TOMASZEWSKA B. Antioxidative defense to lead stress in subcellular compartments of pea root cells. Acta Biochimica Polonica, 2001, 48(3): 687-698.
Crossref Google Scholar
[26]
MA J, ZHOU X M, WANG H. Inhibitory effect of four insecticides on trehelase activity in Plutella xylostella. Chinese Journal of Applied Entomology, 2013, 50(3): 764-769. (in Chinese)
Google Scholar
[27]
WU K K, GU G, LAI R Q, ZHOU T, HAN M. Effects of photoperiod, temperature and humidity on the parasitic ability and reproduction of Aphidius gifuensis (Hymenoptera: Braconidae). Acta Entomologica Sinica, 2022, 65(11): 1488-1497. (in Chinese)
Google Scholar
[28]
LI H B, ZHANG C H, JIA F Z, DAI C G, HU Y. Population rejuvenation of Mythimna separata, a prey of the important natural enemy Arma chinensis. Plant Protection, 2022, 48(1): 127-131, 149. (in Chinese)
Google Scholar
[29]
DAI P L, XU Z Q. Population rejuvenation of mass-rearing Scleroderma guani. Entomological Knowledge, 2007, 44(3): 402-405. (in Chinese)
Google Scholar
[30]
CHEN S. Studies on the rearing technology of Aphidius gifuensis[D]. Yaan: Sichuan Agricultural University, 2015. (in Chinese)
[31]
LIU Q, MA J G, DING F Z, ZHENG D F, XIONG J, ZHANG R C, LI Q. Influence of humidity on reproduction of Aphidius gifuensis. Journal of Anhui Agricultural Sciences, 2015, 43(31): 88-90. (in Chinese)
Google Scholar
[32]
GUO W L. Degradation characteristics and rejuvenation technology of Dastarcus helophoroides Fairmaire[D]. Hefei: Anhui Agricultural University, 2016. (in Chinese)
Scientia Agricultura Sinica
Volume 58 Issue 7,
April 2025
Pages 1344-1354
DOI: 10.3864/j.issn.0578-1752.2025.07.008
Cite this article:
HE J, HAN M, ZHOU T, et al. Effects of Wild Populations for Hybridization and Outdoor Population Exercise on the Parasitic Ability of Aphidius gifuensis and Enzymes Related to the Growth and Development. Scientia Agricultura Sinica, 2025, 58(7): 1344-1354. https://doi.org/10.3864/j.issn.0578-1752.2025.07.008

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Received: 20 November 2024
Accepted: 03 January 2025
Published: 01 April 2025
© 2025 The Journal of Scientia Agricultura Sinica
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