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Publishing Language: Chinese

The Combination of Lipidome and Transcriptome Revealed the Differential Expression Patterns of Lipid Characteristics in Different Muscle Tissues for Nanyang Cattle

YanHao GAO1, TingTing WANG1, WeiWei BAI1, XingJie DU1, Xian LIU2, BenYuan QIN2, Tong FU1, Yu SUN1, TengYun GAO1( ), TianLiu ZHANG1( )
College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046
Animal Husbandry Technology Extension Station of Henan Province, Zhengzhou 450002
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Abstract

【Objective】

The composition and content of intramuscular lipids are important factors affecting flavor and tenderness of beef, and are closely related to beef quality. In this study, by comparing the lipid characteristics and gene expression patterns of longissimus dorsi muscle and tenderloin tissue of Nanyang cattle, the potential candidate genes regulating lipid characteristics of different muscle tissues in Nanyang cattle were identified.

【Method】

The longissimus dorsi muscle and tenderloin tissues of adult Nanyang cattle with the same growth environment and genetic background were selected as experimental materials, and then the lipid profile and gene expression profile of muscle tissue were constructed by gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS) and transcriptome sequencing (RNA-seq) to identify differential lipid molecules (DLMs) and differentially expressed genes (DEGs) between different tissues. Functional enrichment analysis and protein-protein interaction network (PPI) were performed to screen potential candidate genes, and real-time fluorescent quantitative PCR (RT-qPCR) was used to verify their expression levels.

【Result】

In this study, 19 kinds of fatty acids were detected in muscle tissue, among which the content of C18:0, C14:1n5 and C16:1n7 were significantly different between tissues. A total of 2 106 lipid molecules were detected, of which Phosphatidylcholine (PC), Triacylglycerols (TG) and Phosphatidylethanolamine (PE) were the main components. A total of 39 DLMs and 3,424 DEGs were screened between two muscle tissues by difference analysis. According to receiver operating characteristic curve (ROC) analysis, 13 DLMs (e.g. DG(16:0_18:1), DG(18:0_18:1), DG(18:0_18:2)) could be used as potential lipid biomarkers between tissues. PPI and MCODE analysis obtained three core gene modules closely related to lipid metabolism. Pathway enrichment analysis showed that DEGs and DLMs were involved in Inositol phosphate metabolism, Glycerolipid metabolism and Glycerophospholipid metabolism. Integration analysis identified 19 potential candidate genes with different lipid characteristics, among which 7 genes (PLCG2, SYNJ1, LPIN1, DGKZ, DGAT1, LPL and SELENOI) were located in key positions in the pathway, and had direct regulatory effects on DLMs. RT-qPCR showed that the expression trend of six candidate genes was consistent with that of RNA-seq.

【Conclusion】

In this study, 13 potential lipid biomarkers were identified and 19 potential candidate genes were screened for the key metabolic pathways involved in the regulation of lipid characteristics between longissimus dorsi muscle and tenderloin tissue for Nanyang cattle, which provided a theoretical basis for further exploration of the regulatory mechanism for lipid difference formation in Nanyang cattle and molecular breeding for high-quality beef.

Keywords

Nanyang cattlelipid biomarkerslipidometranscriptomecandidate gene

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Scientia Agricultura Sinica
Volume 58 Issue 6,
March 2025
Pages 1239-1258
DOI: 10.3864/j.issn.0578-1752.2025.06.014

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GAO Y, WANG T, BAI W, et al. The Combination of Lipidome and Transcriptome Revealed the Differential Expression Patterns of Lipid Characteristics in Different Muscle Tissues for Nanyang Cattle. Scientia Agricultura Sinica, 2025, 58(6): 1239-1258. https://doi.org/10.3864/j.issn.0578-1752.2025.06.014

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Received: 05 November 2024
Accepted: 20 February 2025
Published: 16 March 2025
© 2025 The Journal of Scientia Agricultura Sinica