AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Scientia Agricultura Sinica Article
PDF (1.8 MB)
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript AI Chat Paper
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Publishing Language: Chinese

Screening and Identification of Pigm-1 Interaction Proteins for Disease Resistance of Rice Blast

YiDan JIN1,2, NiQing HE1, ZhaoPing CHENG1, ShaoJun LIN1, FengHuang HUANG1, KangCheng BAI1,2, Tao ZHANG1,2, WenXiao WANG1,2, MinXiang YU1, DeWei YANG1( )
Institute of Rice, Fujian Academy of Agricultural Sciences, Fuzhou 350018
College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002
Show Author Information

Abstract

【Objective】

Rice blast is one of the most devastating diseases of rice production. A broad-spectrum disease resistance gene Pigm-1 was identified but its functional pathway and interactors are unknown. The screening and identification of key proteins in the Pigm-1 signaling pathway will provide an important theoretical basis for rice disease resistance breeding.

【Method】

In this study, the decoy protein pGBKT7-Pigm-1-CC1-576 vector was constructed to detect the decoy protein self-activation, and the toxicity of the decoy protein was detected by separately transforming the plasmid pGBKT7 and pGBKT7-Pigm-1-CC1-576 into Y2H Gold yeast. The rice disease resistance R protein Pigm-1 was screened by cDNA expression yeast library induced by rice blast fungus. The sequencing results were compared and annotated by Rice Information GateWay (RIGW). The interaction of OsbHLH148 protein was verified by Luc, Co-IP and yeast two-hybrid assays, and the tissue expression of the corresponding gene of the interaction protein OsbHLH148 was analyzed by qRT-PCR.

【Result】

The self-activation test showed that the decoy protein pGBKT7-Pigm-1-CC1-576 did not self-activate when cotransformed with the AD plasmid, and the toxicity analysis showed that the decoy protein had little or no toxicity to yeast cells. A total of 124 proteins that may interact with Pigm-1 were obtained by screening the yeast library, and among these proteins, there are ethylene synthesis related, gibberellin synthesis related, active oxygen species clearly related, enzyme metabolism related, and some function unknown. The interaction between Pigm-1-CC1-576 and OsbHLH148 was verified by Luc, Co-IP and yeast two-hybrid methods. Further analysis showed that OsbHLH148 can be induced by blast fungus infection, and the tissue expression analysis showed that OsbHLH148 expression level was the highest in rice leaves at 6 weeks.

【Conclusion】

In this study, 124 proteins that may interact with Pigm-1 were obtained. One of these proteins, OsbHLH148, was selected and verified to interact with Pigm-1-CC1-576. Suggesting that OsbHLH148 may be involved in Pigm-1 mediated resistance of rice blast.

Keywords

ricerice blastPigm-1interacting proteinOsbHLH148

References

[1]
ELERT E. Rice by the numbers: A good grain. Nature, 2014, 514(7524): S50-S51.
Crossref Google Scholar
[2]
KAMARUDIN S A A, AHMAD F, HASAN N, HISHAM S N, YUSOF S N, ABU HASSAN A, HUSSEIN S, HARUN A R, WAN CHIK W D, MD SAAD M, FAIZAL AZIZI M M, ABD AZIZ SHAMSUDIN N. Whole genome resequencing data and grain quality traits of the rice cultivar Mahsuri and its blast disease resistant mutant line, Mahsuri Mutant. Data in Brief, 2024, 52: 109974.
Crossref Google Scholar
[3]
ZHOU H P, ZHANG F, CHEN K, SHEN C C, ZHU S B, QIU X J, XU J L. Identification of rice blast resistance in Xian and Geng germplasms by genomewide association study. Acta Agronomica Sinica, 2023, 49(5): 1170-1183. (in Chinese)
Crossref Google Scholar
[4]
YANG D W, WANG M, HAN L B, TANG D Z, LI S P. Progress of cloning and breeding application of blast resistance genes in rice and avirulence genes in blast fungi. Chinese Bulletin of Botany, 2019, 54(2): 265-276. (in Chinese)
Google Scholar
[5]
HU K M, QIU D Y, SHEN X L, LI X H, WANG S P. Isolation and manipulation of quantitative trait loci for disease resistance in rice using a candidate gene approach. Molecular Plant, 2008, 1(5): 786-793.
Crossref Google Scholar
[6]
MIAH G, RAFII M Y, ISMAIL M R, PUTEH A B, RAHIM H A, ASFALIZA R, LATIF M A. Blast resistance in rice: A review of conventional breeding to molecular approaches. Molecular Biology Reports, 2013, 40(3): 2369-2388.
Crossref Google Scholar
[7]
AN Z S, LIU G L, MEI H W, LI L T, WANG J H, LUO L J. Improving blast resistance of parental lines of drought resistant hybrid rice by marker-assisted selection. Molecular Plant Breeding, 2010, 8(6): 1172-1176. (in Chinese)
Google Scholar
[8]
ELLUR R K, KHANNA A, YADAV A, PATHANIA S, RAJASHEKARA H, SINGH V K, GOPALA KRISHNAN S, BHOWMICK P K, NAGARAJAN M, VINOD K K, PRAKASH G, MONDAL K K, SINGH N K, VINOD PRABHU K, SINGH A K. Improvement of Basmati rice varieties for resistance to blast and bacterial blight diseases using marker assisted backcross breeding. Plant Science, 2016, 242: 330-341.
Crossref Google Scholar
[9]
DIAO Z J, CHEN L Z, WANG X, LU L, LIU Y, ZHANG J, XIA N, TANG D Z, LI S P. The mechanism of cystathionine-β-synthase OsCBSX4 in rice blast resistance. Scientia Agricultura Sinica, 2024, 57(23): 4593-4606. doi: 10.3864/j.issn.0578-1752.2024.23.002. (in Chinese)
Google Scholar
[10]
KOU Y J, SHI H B, QIU J H, TAO Z, WANG W M. Effectors and environment modulating rice blast disease: from understanding to effective control. Trends in Microbiology, 2024, 32(10): 1007-1020.
Crossref Google Scholar
[11]
XIAO N, WU Y Y, LI A H. Strategy for use of rice blast resistance genes in rice molecular breeding. Rice Science, 2020, 27(4): 263-277.
Crossref Google Scholar
[12]
SU J, WANG W J, HAN J L, CHEN S, WANG C Y, ZENG L X, FENG A Q, YANG J Y, ZHOU B, ZHU X Y. Functional divergence of duplicated genes results in a novel blast resistance gene Pi50 at the Pi2/9 locus. Theoretical and Applied Genetics, 2015, 128(11): 2213-2225.
Crossref Google Scholar
[13]
DENG Y W, ZHAI K R, XIE Z, YANG D Y, ZHU X D, LIU J Z, WANG X, QIN P, YANG Y Z, ZHANG G M, et al. Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance. Science, 2017, 355(6328): 962-965.
Crossref Google Scholar
[14]
ZHAI K R, DENG Y W, LIANG D, TANG J, LIU J, YAN B X, YIN X, LIN H, CHEN F D, YANG D Y, XIE Z, LIU J Y, LI Q, ZHANG L, HE Z H. RRM transcription factors interact with NLRs and regulate broad-spectrum blast resistance in rice. Molecular Cell, 2019, 74(5): 996-1009.
Crossref Google Scholar
[15]
YANG D, LI S, LU L, FANG J, WANG W, CUI H, TANG D. Identification and application of the Pigm-1 gene in rice disease resistance breeding. Plant Biology, 2020, 22(6): 1022-1029.
Crossref Google Scholar
[16]
YANG D W, HE N Q, HUANG F H. Pyramiding Pigm-1 and Xa23 genes in rice (Oryza sativa L.) by marker-assisted selection. Journal of Northwest A & F University (Natural Science Edition), 2023, 51(11): 37-45. (in Chinese)
Google Scholar
[17]
HE N Q, YANG D W, ZHENG X H, HUANG F H, CHENG C P, YE N. Improving blast resistance of R20 by molecular marker-assisted selection of Pigm-1 gene. Journal of Nuclear Agricultural Sciences, 2022, 36(2): 245-250. (in Chinese)
Google Scholar
[18]
TAMELING W I L, TAKKEN F L W. Resistance proteins: Scouts of the plant innate immune system. European Journal of Plant Pathology, 2008, 121(3): 243-255.
Crossref Google Scholar
[19]
SLOOTWEG E, ROOSIEN J, SPIRIDON L N, PETRESCU A J, TAMELING W, JOOSTEN M, POMP R, VAN SCHAIK C, DEES R, BORST J W, SMANT G, SCHOTS A, BAKKER J, GOVERSE A. Nucleocytoplasmic distribution is required for activation of resistance by the potato NB-LRR receptor Rx1 and is balanced by its functional domains. The Plant Cell, 2010, 22(12): 4195-4215.
Crossref Google Scholar
[20]
QI D, INNES R W. Recent advances in plant NLR structure, function, localization, and signaling. Frontiers in Immunology, 2013, 4: 348.
Crossref Google Scholar
[21]
ZHAO Y L, CAO X, ZHONG W H, ZHOU S K, LI Z B, AN H, LIU X H, WU R F, BOHORA S, WU Y, LIANG Z Y, CHEN J H, YANG X, ZHOU G H, ZHANG T. A viral protein orchestrates rice ethylene signaling to coordinate viral infection and insect vector-mediated transmission. Molecular Plant, 2022, 15(4): 689-705.
Crossref Google Scholar
[22]
DE VLEESSCHAUWER D, SEIFI H S, FILIPE O, HAECK A, HUU S N, DEMEESTERE K, HÖFTE M. The DELLA protein SLR1 integrates and amplifies salicylic acid- and jasmonic acid-dependent innate immunity in rice. Plant Physiology, 2016, 170(3): 1831-1847.
Crossref Google Scholar
[23]
HOU H N, FANG J B, LIANG J H, DIAO Z J, WANG W, YANG D W, LI S P, TANG D Z. OsExo70B1 positively regulates disease resistance to Magnaporthe oryzae in rice. International Journal of Molecular Sciences, 2020, 21(19): 7049.
Crossref Google Scholar
[24]
SEO J S, JOO J, KIM M J, KIM Y K, NAHM B H, SONG S I, CHEONG J J, LEE J S, KIM J K, CHOI Y D. OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice. The Plant Journal, 2011, 65(6): 907-921.
Crossref Google Scholar
[25]
HAN J L, WANG X Y, WANG F P, ZHAO Z, LI G S, ZHU X Y, SU J, CHEN L T. The fungal effector avr-pita suppresses innate immunity by increasing COX activity in rice mitochondria. Rice, 2021, 14(1): 12.
Crossref Google Scholar
[26]
WANG R Y, YOU X M, ZHANG C Y, FANG H, WANG M, ZHANG F, KANG H X, XU X, LIU Z, WANG J Y, et al. An ORFeome of rice E3 ubiquitin ligases for global analysis of the ubiquitination interactome. Genome Biology, 2022, 23(1): 154.
Crossref Google Scholar
[27]
XU R R, SONG F M, ZHENG Z. OsBISAMT1, a gene encoding S-adenosyl-L-methionine: Salicylic acid carboxyl methyltransferase, is differentially expressed in rice defense responses. Molecular Biology Reports, 2006, 33(3): 223-231.
Crossref Google Scholar
[28]
LIU H F, LU C C, LI Y, WU T, ZHANG B G, LIU B Y, FENG W J, XU Q, DONG H S, HE S Y, CHU Z H, DING X H. The bacterial effector AvrRxo1 inhibits vitamin B6 biosynthesis to promote infection in rice. Plant Communications, 2022, 3(3): 100324.
Crossref Google Scholar
[29]
ZOU X, LIU L, HU Z B, WANG X K, ZHU Y C, ZHANG J L, LI X F, KANG Z Y, LIN Y J, YIN C X. Salt-induced inhibition of rice seminal root growth is mediated by ethylene-jasmonate interaction. Journal of Experimental Botany, 2021, 72(15): 5656-5672.
Crossref Google Scholar
[30]
YANG D W, WANG X, ZHENG X X, XIANG X Q, CUI H T, LI S P, TANG D Z. Functional studies of rice blast resistance related gene OsSAMS1. Acta Agronomica Sinica, 2022, 48(5): 1119-1128.
Crossref Google Scholar
[31]
ZHANG M X, ZHAO R R, HUANG K, HUANG S Z, WANG H T, WEI Z Q, LI Z, BIAN M D, JIANG W Z, WU T, DU X L. The OsWRKY63–OsWRKY76–OsDREB1B module regulates chilling tolerance in rice. The Plant Journal, 2022, 112(2): 383-398.
Crossref Google Scholar
[32]
YOKOTANI N, SATO Y, TANABE S, CHUJO T, SHIMIZU T, OKADA K, YAMANE H, SHIMONO M, SUGANO S, TAKATSUJI H, KAKU H, MINAMI E, NISHIZAWA Y. WRKY76 is a rice transcriptional repressor playing opposite roles in blast disease resistance and cold stress tolerance. Journal of Experimental Botany, 2013, 64(16): 5085-5097.
Crossref Google Scholar
[33]
SEO Y S, CHERN M, BARTLEY L E, HAN M, JUNG K H, LEE I, WALIA H, RICHTER T, XU X, CAO P J, et al. Towards establishment of a rice stress response interactome. PLoS Genetics, 2011, 7(4): e1002020.
Crossref Google Scholar
Scientia Agricultura Sinica
Volume 58 Issue 6,
March 2025
Pages 1043-1051
DOI: 10.3864/j.issn.0578-1752.2025.06.001

{{item.num}}

Comments on this article

Go to comment

< Back to all reports

Review Status: {{reviewData.commendedNum}} Commended , {{reviewData.revisionRequiredNum}} Revision Required , {{reviewData.notCommendedNum}} Not Commended Under Peer Review

Review Comment

Cite this Report
Close
Close
Cite this article:
JIN Y, HE N, CHENG Z, et al. Screening and Identification of Pigm-1 Interaction Proteins for Disease Resistance of Rice Blast. Scientia Agricultura Sinica, 2025, 58(6): 1043-1051. https://doi.org/10.3864/j.issn.0578-1752.2025.06.001

124

Views

3

Downloads

0

Crossref

0

Scopus

0

CSCD

Altmetrics
Received: 26 December 2024
Accepted: 20 January 2025
Published: 16 March 2025
© 2025 The Journal of Scientia Agricultura Sinica