AI Chat Paper
Discover the SciOpen Platform and Achieve Your Research Goals with Ease.
Search articles, authors, keywords, DOl and etc.
{{expandStatus?'Exit ':''}}Advanced Search
The purpose of this study was to optimize ultrasound-targeted microbubble destruction (UTMD) on RAGE plasmid transfection in human coronary artery endothelial cells (HCAECs) and improve gene transfection efficiency in vitro.
SonoVue microbubble suspension was prepared and mixed with HCAECs, while the cells were adherent and suspended, respectively. After RAGE plasmids being added, they were exposed to ultrasonic irradiation for 10, 20, and 30 s by a therapeutic US machine with 0.4W, respectively. The samples with adherent HCAECs (adherent group) were irradiated directly, while the samples with suspended HCAECs (suspended group) were irradiated via the water. The combined effect of ultrasound and microbubble on RAGE plasmid transfection in HCAECs was evaluated by detecting protein expression of RAGE by western blot. In addition, the viability of the HCAECs was analyzed by CCK8 in order to explore the optimal transfection condition.
In suspension group, compared with control, the expression of RAGE was gradually increased from 5 to 20s, and decreased from 20 to 30s. The expression of RAGE peaked in 20s and indicated statistical significance. However, compared with the control, the expression of RAGE did not significantly increase with prolonged ultrasound irradiation in the adherent group. On the other hand, viability of the HCAECs did not decrease significantly with extended exposure time in both groups.
UTMD represents an efficient and safe method for the transfection of cells in suspension and optimal exposure.
Du X, Wang J, Zhou Q, Zhang L, Wang S, Zhang Z, et al. Advanced physical techniques for gene delivery based on membrane perforation. Drug Deliv 2018;25: 1516-25.
Hecker M, Wagner AH. Transcription factor decoy technology: A therapeutic update. Biochem Pharmacol 2017;144:29-34.
Rinaldi L, Folliero V, Palomba L, Zannella C, Isticato R, Di Francia R, et al. Sonoporation by microbubbles as gene therapy approach against liver cancer. Oncotarget 2018;9:32182-90.
Yang C, Li B, Yu J, Yang F, Cai K, Chen Z. Ultrasound microbubbles mediated miR-let-7b delivery into CD133+ovarian cancer stem cells. Biosci Rep 2018;38: BSR20180922.
Hu B, Jiang N, Zhou Q, Cao S, Cao S, Zhang B, et al. Stable cavitation using acoustic phase-change dodecafluoropentane nanoparticles for coronary micro-circulation thrombolysis. Int J Cardiol 2018;272:1-6.
Nguyen AH, Detty SQ, Agrewal DK. Clinical implications of high-mobility group box-1 (HMGB1) and the receptor for advanced glycation end-products (RAGE). Anticancer Res 2017;37:1-7.
Ma WQ, Qu QR, Zhao Y, Liu NF. Association of RAGE gene Gly82Ser polymorphism with coronary artery disease and ischemic stroke: A systematic review and meta-analysis. Medicine 2016;95:e5593.
Xu P, Gul-Uludag H, Ang WT, Yang X, Huang M, Marquez-Curtis L, et al. Low-intensity pulsed ultrasound-mediated stimulation of hematopoietic stem/progenitor cell viability, proliferation and differentiation in vitro. Biotechnol Lett 2012; 34:1965–73.
Shi C, Zhang Y, Yang H, Dong T, Chen Y, Xu Y, et al. Combined effect of ultrasound/SonoVue microbubble on CD4(+)CD25(+) regulatory T cells viability and optimized parameters for its transfection. Ultrasonics 2015;62:97-102.
Duvshani-Eshet M, Adam D, Machluf M. The effects of albumin-coated microbubbles in DNA delivery mediated by therapeutic ultrasound. J Control Release 2006;12:156-66.
Sun RR, Noble ML, Sun SS, Song S, Miao CH. Development of therapeutic microbubbles for enhancing ultrasound-mediated gene delivery. J Control Release 2014;182:111-20.
Stride E. Physical principles of microbubbles for ultrasound imaging and therapy. Front Neurol Neurosci. Front Neurol Neurosci 2015; 36:11-22.
Xie R, Xu T, Zhu J, Wei X, Zhu W, Li L, et al. The combination of Glycolytic inhibitor 2-Deoxyglucose and microbubbles increases the effect of 5-aminolevulinic acid-sonodynamic therapies in liver cancer cells. Ultrasound Med Biol 2017;43: 2640-50.
Zhou Y, Gu H, Xu Y, Li F, Kuang S, Wang Z, et al. Targeted antiangiogenesis gene therapy using targeted cationic microbubbles conjugated with CD105 antibody compared with untargeted cationic and neutral microbubbles. Theranostics 2015;5: 399-417.
Cai W, Lv W, Feng Y, Yang H, Zhang Y, Yang G, et al. The therapeutic effect in gliomas of nanobubbles carrying siRNA combined with ultrasound-targeted destruction. Int J Namomedicine 2018;13:6791-807.
Tu J, Zhang H, Yu J, Liufu C, Chen Z. Ultrasound-mediated microbubble destruction: a new method in cancer immunotherapy. Onco Targets Ther 2018;11:5763-75.
Toedebusch R, Belenchia A, Pulakat L. Cell-specific protective signaling induced by the novel AT2R-Agonist NP-6A4 on human endothelial and smooth muscle cells. Front Pharmacol 2018;9:928.
Turner AW, Wong D, Dreisbach CN, Miller CL. GWAS reveal targets in vessel wall pathways to treat coronary artery disease. Front Cardiovasc Med 2018;5:72.
Li Z, Huang H, Huang L, Du L, Sun Y, Duan Y. Prevention of oxidized low-density lipoprotein-induced endothelial cell injury by DA-PLGA-PEG-cRGD nanoparticles combined with ultrasound. Int J Mol Sci 2017; 18:E815.
Reslan L, Mestas JL, Herveau S, Béra JC, Dumontet C. Transfection of cells in suspension by ultrasound cavitation. J Control Release 2010; 142:251-258.
Lo CW, Desjouy C, Chen SR, Lee JL, Inserra C, Bera JC, et al. Stabilizing in vitro ultrasound-mediated gene transfection by regulating. Ultrason Sonochem 2014;21: 833-9.
Moosavi Nejad S, Hosseini SH, Aklyama H, Tachibana K. Optical observation of cell sonoporation with low intensity ultrasound. Biochem Biophis Res Commun 2011; 413:218-23.
Kodama T, Tomita Y, Koshiyama K, Blomley MJ. Transfection effect of microbubbles on cells in superposed ultrasound waves and behavior of cavitation bubble. Ultrasound Med Biol 2016; 32:905-14.
Fan Z, Chen D, Deng CX. Improving ultrasound gene transfection efficiency by controlling ultrasound excitation of microbubbles. J Control Release 2013;170: 401-13.
{{item.fileName}}
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
京公网安备11010802044758号
Comments on this article