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Basic Study | Publishing Language: Chinese | Open Access

Study on lncRNA ADAMTS9-AS2 promoting invasion and metastasis of salivary adenoid cystic carcinoma

Donghui WU1, Yunying ZHU1, Jianqiang LIANG1, Zhaoyu LIN2, Jinsong LI2( )
Department of Oral Surgery, Stomatology hospital of Haizhu district, Guangzhou 510220, China
Department of Oral & Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510220, China
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Abstract

Objective

To investigate the role of lncRNAs in the invasion and metastasis of salivary adenoid cystic carcinoma (SACC) cells.

Methods

With SACC-LM as the experimental group and SACC83 as the control group, lncRNA chips were used to screen the differentially expressed lncRNAs. The differentially expressed lncRNAs were further verified by real-time quantitative RT-PCR (qRT-PCR). The invasion and migration abilities of the adenoid cystic carcinoma cell lines before and after transfection with lncRNA siRNAs were detected by invasion and migration experiments. The clinicopathological features and prognosis of patients with different expression of lncRNAs and SACC were analyzed.

Results

The microarray showed that ADAMTS9-AS2 was highly expressed in the SACC-LM cells. Real-time quantitative RT-PCR further confirmed that ADAMTS9-AS2 was significantly upregulated in the SACC-LM cells. Invasion and migration experiments showed that the invasion and migration were significantly reduced after the expression level of ADAMTS9-AS2 was downregulated (P < 0.001). Analysis of the clinicopathological data showed that ADAMTS9-AS2 was highly expressed in SACC. High expression of ADAMTS9-AS2 was associated with poor prognosis and a high tumor metastasis rate in SACC patients.

Conclusion

High expression of ADAMTS9-AS2 promotes the migration and invasion of SACC cells. ADAMTS9-AS2 is upregulated in the SACC tissues and is related to a high metastasis rate and poor prognosis.

Keywords

long noncoding RNAlncRNA ADAMTS9-AS2salivary adenoid cystic carcinomaadenoid cystic carcinoma cell line with high metastasislncRNA expression microarrayinvasionmetastasisRNA interferenceepigenetics
CLC number: R78 Document code: A Article ID: 2096-1456(2020)04-0214-05

References

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Journal of Prevention and Treatment for Stomatological Diseases
Volume 28 Issue 4,
April 2020
Pages 214-218
DOI: 10.12016/j.issn.2096-1456.2020.04.002

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Cite this article:
WU D, ZHU Y, LIANG J, et al. Study on lncRNA ADAMTS9-AS2 promoting invasion and metastasis of salivary adenoid cystic carcinoma. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(4): 214-218. https://doi.org/10.12016/j.issn.2096-1456.2020.04.002

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Received: 17 July 2019
Revised: 07 November 2019
Published: 20 April 2020
© 2020 by Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases

This article is licensed under a Creative Commons Attribution 4.0 International License.