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Basic Study | Publishing Language: Chinese | Open Access

Influence of concentrations of CaCl2 on hemolytic activity of candida strains

Lei WAN1, Haibin LU1( ), Gang LUO2
Department of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou 510280, China
Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medicial University, Guangzhou 510140, China
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Abstract

Objective

To investigate the influence of different concentrations of CaCl2 on the hemolytic activity of candida strains.

Methods

A total of 25 candida strains were selected, including 21 candida strains recovered from clinical specimens, and 4 reference strains of C. albicans (ATCC 90028) (American Type Culture Collection, Manassas, VA, USA), C. glabrata (ATCC 90030), C. krusei (ATCC 6258), and C. tropicalis (ATCC 13803) respectively. CaCl2 was added at concentrations of 0%, 0.5%, 1% and 2.5 g % (wt/vol). Plates were cultured at 37 ℃ in a 5% CO2 atmosphere for 24-72 h, and the hemolytic index (Hi) was analyzed.

Results

Comparsions of the hemolytic indices among the groups treated with 0.5% CaCl2 (Hi = 2.247 ± 0.079), 1% CaCl2 (Hi = 2.013 ± 0.092), 2.5% CaCl2 (Hi = 2.150 ± 0.066) and the control (Hi = 2.749 ± 0.103) reached statistical significance (P＜0.001).

Conclusion

CaCl2 may produce a decrease activity in the hemolysis of candida species.

Keywords

Candida speciesVirulence factorsHemolytic activityElectrolytesCaCl2
CLC number: R781.5 Document code: A Article ID: 2096-1456(2017)08-0494-04

References

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Journal of Prevention and Treatment for Stomatological Diseases
Volume 25 Issue 8,
August 2017
Pages 494-497
DOI: 10.12016/j.issn.2096-1456.2017.08.004

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Cite this article:
WAN L, LU H, LUO G. Influence of concentrations of CaCl2 on hemolytic activity of candida strains. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(8): 494-497. https://doi.org/10.12016/j.issn.2096-1456.2017.08.004

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Received: 05 April 2017
Revised: 02 May 2017
Published: 20 August 2017
© 2017 by Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases

This article is licensed under a Creative Commons Attribution 4.0 International License.