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Letter | Open Access

From elite germplasm to transformation platform: Breaking recalcitrance in Tartary buckwheat

Zhen Wang1,*, Tong Su1,*, Kaixuan Zhang1, Yuqi He1, Zhirong Wang1, Alexander Betekhtin2, Meiliang Zhou1( )
State Key Laboratory of Crop Gene Resources and Breeding/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Katowice 40-032, Poland

* These authors contributed equally to this study.

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Highlights

• An elite germplasm, G253, with superior morphogenic callus induction and proliferation capacity was identified.

• A stable Agrobacterium-mediated transformation platform enabling transgenic plant generation was established.

• An efficient protoplast transient transformation system was developed from the morphogenic callus.

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Journal of Integrative Agriculture (JIA)
Volume 25 Issue 7,
July 2026
Pages 3090-3093
DOI: 10.1016/j.jia.2026.03.022

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Cite this article:
Wang Z, Su T, Zhang K, et al. From elite germplasm to transformation platform: Breaking recalcitrance in Tartary buckwheat. Journal of Integrative Agriculture (JIA), 2026, 25(7): 3090-3093. https://doi.org/10.1016/j.jia.2026.03.022

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Received: 09 January 2026
Revised: 03 February 2026
Accepted: 06 February 2026
Published: 10 March 2026
© 2026 CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer review under responsibility of Editorial Board of Journal of Integrative Agriculture.