Highlights
• A visual assay of Langya henipavirus (LayV) nucleic acids was developed based on recombinase polymerase amplification technology (RPA), combined with an immunochromatographic test device.
• The detection limit of our assay reaches 1.22 copies μL-1, with no observed cross-reactivity with other henipaviruses.
• This assay is well-suited for the requirements of point-of-care and field detection and has the potential for broader application.
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