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Full Length Article | Open Access

Ce6-GFFY is a novel photosensitizer for colorectal cancer therapy

Wei Qiaoa,1, Shuxin Lib,1, Linna Luoa,1, Meiling Chenb,c, Xiaobin Zhengb,c, Jiacong Yeb, Zhaohui Lianga, Qiaoli Wangb, Ting Hua, Ling Zhoub, Jing Wangb, Xiaosong Ged, Guokai Fengb, Fang Hue, Rongbin Liuf,g( ), Jianjun Lia( ), Jie Yangb( )
Department of Endoscopy, State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, China
State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, China
Department of Nuclear Medicine, State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, China
Department of Oncology, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, China
Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Biomaterials Research Center, School of Biomedical Engineering, Southern Medical University, Guangzhou, Guangdong 510515, China
Department of Ultrasound, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510120, China
Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510120, China

1 These authors contributed equally to this work.

Peer review under the responsibility of the Genes & Diseases Editorial Office, in alliance with the Association of Chinese Americans in Cancer Research (ACACR, Baltimore, MD, USA)

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Abstract

Photodynamic therapy is an “old” strategy for cancer therapy featuring clinical safety and rapid working, but suitable photosensitizers for colorectal cancer therapy remain lacking. This study synthesized a novel photosensitizer termed Ce6-GFFY based on a self-assembling peptide GFFY and a photo-responsive molecule chlorin e6 (Ce6). Ce6-GFFY forms macroparticles with a diameter of ∼160 nm and possesses a half-life of 10 h, as well as an ideal tumor-targeting ability in mouse models. Ce6-GFFY effectively penetrates cells and generates numerous reactive oxygen species upon 660 nm laser irradiation. The reactive oxygen species promotes the accumulation of cytotoxic T cells and decrease of myeloid-derived suppressor cells in the tumor microenvironment through immunogenic cell death, thus prohibiting the growth of both primary and metastatic tumors after once treatment. This study not only provides a strategy for photosensitizer development but also confirms a promising application of Ce6-GFFY for colorectal cancer therapy.

Keywords

Anti-tumor immunityCe6-GFFYColorectal cancerNovel photosensitizerPhotodynamic therapy

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Genes & Diseases
Volume 12 Issue 2,
March 2025
Article number: 101441
DOI: 10.1016/j.gendis.2024.101441
Cite this article:
Qiao W, Li S, Luo L, et al. Ce6-GFFY is a novel photosensitizer for colorectal cancer therapy. Genes & Diseases, 2025, 12(2): 101441. https://doi.org/10.1016/j.gendis.2024.101441

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Received: 17 October 2023
Revised: 10 July 2024
Accepted: 21 October 2024
Published: 28 October 2024
© 2024 The Authors.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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