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Rapid Communication | Open Access

Precise and efficient insertion of A673T mutation in APP gene using MSYM

Qing Xiaa,b,1Zijie Liua,1Xinyu Yanga,1Jiaying XiaoaXue ZhaoaYu ZhaoaFeifei ZhengaFangliang GeaKe YeaLulu LiuaDayong Wanga,c,d,e( )Xu Gaoa,c,d,e( )
Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang 150000, China
Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
Basic Medical Institute, Heilongjiang Medical Science Academy, Harbin, Heilongjiang 150000, China
Translational Medicine Center of Northern China, Harbin, Heilongjiang 150000, China
Key Laboratory of Heilongjiang Province for Genetically Modified Animals, Harbin Medical University, Harbin, Heilongjiang 150000, China

1 These authors contributed equally to this work.

Peer review under responsibility of Chongqing Medical University.

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References

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Jonsson T, Atwal JK, Steinberg S, et al. A mutation in APP protects against Alzheimer's disease and age-related cognitive decline. Nature. 2012;488(7409):96-99.

2

Tremblay G, Rousseau J, Mbakam CH, Tremblay JP. Insertion of the Icelandic mutation (A673T) by prime editing: a potential preventive treatment for familial and sporadic Alzheimer's disease. Crispr J. 2022;5(1):109-122.

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Richardson CD, Ray GJ, DeWitt MA, Curie GL, Corn JE. Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA. Nat Biotechnol. 2016;34(3):339-344.

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Lin S, Staahl BT, Alla RK, Doudna JA. Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. Elife. 2014;3:e04766.

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Maruyama T, Dougan SK, Truttmann MC, Bilate AM, Ingram JR, Ploegh HL. Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining. Nat Biotechnol. 2015;33(5):538-542.

Genes & Diseases
Article number: 101154
Cite this article:
Xia Q, Liu Z, Yang X, et al. Precise and efficient insertion of A673T mutation in APP gene using MSYM. Genes & Diseases, 2024, 11(6): 101154. https://doi.org/10.1016/j.gendis.2023.101154

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Received: 20 July 2023
Published: 27 October 2023
© 2023 The Authors.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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