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Research Article | Open Access

Nuciferine relieves type 2 diabetes mellitus via enhancing GLUT4 expression and translocation

Tongxi Zhoua, Guanjun Songb, Di Tianb, Qinghua Liub, Jinhua Shenb, Xinzhou Yanga( ), Ping Zhaob( )
School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, China
Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection and Application of Special Plants in the Wuling Area of China, College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

Nuciferine contained in lotus leaves have been confirmed to have the effect of ameliorating hyperlipemia and hyperglycemia. A laser scanning confocal microscope was used to track the translocation of glucose transporter 4 (GLUT4) in L6 cells and the changes in intracellular Ca2+ levels in real time, and related protease inhibitors combined with western blotting were used to explore the mechanism of nuciferine. Meanwhile, KK-Ay mice, the spontaneous type 2 diabetic mice, were used to evaluate the in vivo activity of nuciferine. In this study, the in vitro studies indicated that nuciferine-induced GLUT4 translocation was regulated by G protein-PLC-PKC and AMPK pathways and nuciferine-enhanced intracellular Ca2+ was mediated by G protein-PLC-IP3-IP3R pathway, the increase in intracellular Ca2+ caused by nuciferine was not directly related to GLUT4 translocation, but both promote glucose uptake. The in vivo results suggested that nuciferine ameliorated weight gain induced by high-fat diet, abnormal lipid metabolism and the symptoms of insulin resistance in KK-Ay diabetic mice. Western blot results suggested that nuciferine increased AMPK and PKC phosphorylation levels in skeletal muscle and liver, and enhanced GLUT4 expression in skeletal muscle. Taken together, this research showed that nuciferine has the non-negligible potential in the treatment of type 2 diabetes mellitus.

Keywords

NuciferineType 2 diabetes mellitusglucose transporter 4 (GLUT4)

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Food Science and Human Wellness
Volume 12 Issue 6,
November 2023
Pages 2040-2051
DOI: 10.1016/j.fshw.2023.03.020
Cite this article:
Zhou T, Song G, Tian D, et al. Nuciferine relieves type 2 diabetes mellitus via enhancing GLUT4 expression and translocation. Food Science and Human Wellness, 2023, 12(6): 2040-2051. https://doi.org/10.1016/j.fshw.2023.03.020

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Received: 08 May 2021
Revised: 27 May 2021
Accepted: 03 July 2021
Published: 04 April 2023
© 2023 Beijing Academy of Food Sciences.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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