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Research Article | Open Access

Carboxymethylation of the polysaccharide from the fermentation broth of Marasmius androsaceus and its antidepressant mechanisms

Yu Qiua,1, Yan Sua,1, Jia Songa( ), Fangming Moua, Jia Goua, Xiaoqi Genga, Xinyue Lia, Zhiqiang Nieb, Jianxin Wangc,d, Yu Zhenga( ), Min Wanga( )
State Key Laboratory of Food Nutrition and Safety, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China
Key Laboratory of Chemical Biology and Molecular Engineering, Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China
Wisconsin Center for NanoBioSystems, School of Pharmacy, University of Wisconsin-Madison, Madison, WI 53706, USA
Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, Madison, WI 53706, USA

1 The authors contributed equally to this article.

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

To investigate the structure-activity relationship of polysaccharide and obtain a better antidepressant polysaccharide, the antidepressant-like activity of a carboxymethyl polysaccharide (C-MEPS2) subjected to submerged fermentation was systematically studied. PC12-H cell and Kunming mice were used to investigate the differences and their mechanism in the antidepressant effects of C-MEPS2 and MEPS2. Cell experiments have showed that C-MEPS2 has a better antidepressant effect than MEPS2. C-MEPS2 could exert antidepressant effects related to catecholamine synthesis with specific sites of TH, D2DR, and P-CAMKII. In addition, C-MEPS2 could repair the Res-induced damage in PC12-H cell, stabilize the mitochondrial membrane potential and regulate intracellular Ca2+ concentration, thus reducing cell apoptosis caused by RES. Antagonists common dosing experiments on animals further proved that CMEPS2 could significantly improve the antidepressant effect of derivatives without affecting the antidepressant mechanism of MEPS2. It is speculated that it may be related to carboxymethylated modification.

Keywords

Carboxymethyl polysaccharideAnti-depressantCatecholamine

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Food Science and Human Wellness
Volume 12 Issue 6,
November 2023
Pages 2417-2427
DOI: 10.1016/j.fshw.2023.03.009
Cite this article:
Qiu Y, Su Y, Song J, et al. Carboxymethylation of the polysaccharide from the fermentation broth of Marasmius androsaceus and its antidepressant mechanisms. Food Science and Human Wellness, 2023, 12(6): 2417-2427. https://doi.org/10.1016/j.fshw.2023.03.009

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Received: 29 December 2021
Revised: 25 April 2022
Accepted: 23 May 2022
Published: 04 April 2023
© 2023 Beijing Academy of Food Sciences.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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