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Research Article | Open Access

De novo transcriptome assembly of Aureobasidium melanogenum CGMCC18996 to analyze the β-poly(L-malic acid) biosynthesis pathway under the CaCO3 addition

Genan Wanga,b, Haisong Yina,b,c, Tingbin Zhaod, Donglin Yanga,b, Shiru Jiaa,b( ), Changsheng Qiaoa,b,d( )
Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science and Technology), Ministry of Education, Tianjin 300457,China
Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China
School of Bioengineering, Tianjin Modern Vocational Technology College, Tianjin 300350, China
Tianjin Huizhi Biotrans Bioengineering Co., Ltd., Tianjin 300457, China
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Abstract

β-Poly(L-malic acid) (PMLA) is a water-soluble biopolymer used in food, medicine and other industries. To date, the biosynthesis pathway of PMLA has not been fully elucidated. In this study, we sequenced the transcriptome of strain Aureobasidium melanogenum under 20 g/L CaCO3 addition. The resulting sequencing reads were assembled and annotated for the differentially expressed genes (DEGs) analysis and novel transcripts identification. The result indicated that with the CaCO3 addition, the tricarboxylic cycle (TCA) cycle and glyoxylate pathway were up-regulated, and it also found that a non-ribosomal peptide synthetase (NRPS) like protein was highly expressed. The DEGs analysis showed a high expression level of malate dehydrogenase (MDHC) and phosphoenolpyruvate carboxykinase (PCKA) in the CaCO3 group, which indicated a cytosolic malate activity. We speculated that the malate should be transported to or synthesized in the cytoplasm, which was then polymerized to PMLA by the NRPS-like protein, accompanied by the up-regulated TCA cycle providing ATP for the polymerization. Depending on the analysis, we assumed that an NRPS-like protein, the TCA cycle, and the cytosolic malate together are contributing to the PMLA biosynthesis.

Keywords

De novo transcriptome analysisβ-Poly(L-malic acid)Aureobasidium melanogenum

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Food Science and Human Wellness
Volume 12 Issue 4,
July 2023
Pages 1248-1256
DOI: 10.1016/j.fshw.2022.10.007
Cite this article:
Wang G, Yin H, Zhao T, et al. De novo transcriptome assembly of Aureobasidium melanogenum CGMCC18996 to analyze the β-poly(L-malic acid) biosynthesis pathway under the CaCO3 addition. Food Science and Human Wellness, 2023, 12(4): 1248-1256. https://doi.org/10.1016/j.fshw.2022.10.007

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Received: 01 February 2021
Revised: 16 March 2021
Accepted: 28 April 2021
Published: 18 November 2022
© 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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