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Research Article | Open Access

Immunomodulatory effect of ethanol-soluble oligopeptides from Atlantic cod (Gadus morhua)

Zhen Yuana, Meilian Yanga, Dongyang Zhua, Di Wua, Shuzhen Chenga,b, Chao Wua, Hesham R. El-Seedic, Ming Dua ( )
School of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
Pharmacognosy Group, Department of Medicinal Chemistry, Uppsala University, Biomedical Centre, Uppsala 75123, Sweden

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

There are many active substances in Atlantic cod (Gadus morhua) explaining the variety of biological activities. In order to study the immunomodulatory activity and the mechanism of Atlantic cod peptides at the cellular level. In this study, cod peptides were isolated by 80 % ethanol extraction method, the isolated ethanol-soluble cod peptides (CP-ES) were investigated and their immunomodulatory activity was verified. Additionally, CP-ES showed lower molecular weight and more hydrophobic amino acids. CP-ES could promote the proliferation of spleen lymphocytes and T lymphocytes in mice, suggesting that CP-ES may regulate adaptive immunity. It promoted the release of NO and the expression of iNOS, TNF-α, IL-6 and IL-1β genes in macrophages, suggesting that CP-ES may regulate innate immunity. CP-ES could promote the expression of TLR2 gene, and the peptides identified in CP-ES were docked with TLR2 to predict the peptides playing a major role in CP-ES. These results suggested that CP-ES may regulate the immune activity of both innate and adaptive lines.

Keywords

Gadus morhuaImmunomodulationPeptidesTLR2Molecular docking

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Food Science and Human Wellness
Volume 12 Issue 4,
July 2023
Pages 1192-1203
DOI: 10.1016/j.fshw.2022.10.002
Cite this article:
Yuan Z, Yang M, Zhu D, et al. Immunomodulatory effect of ethanol-soluble oligopeptides from Atlantic cod (Gadus morhua). Food Science and Human Wellness, 2023, 12(4): 1192-1203. https://doi.org/10.1016/j.fshw.2022.10.002

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Received: 30 January 2022
Revised: 17 February 2022
Accepted: 04 March 2022
Published: 18 November 2022
© 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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