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Research Article | Open Access

Evaluation of allergenic protein profiles in three Chinese high-oleic acid peanut cultivars using NanoLC-Orbitrap mass spectrometry

Xinke Yanga, Haochen Baia, Lijun Yina, Jin Wangb, Wentong Xuea( ), Xin Jiaa( )
College of Food Science and Nutritional Engineering, Beijing Key Laboratory of Functional Food from Plant Resources, China Agricultural University, Beijing 100083, China
The Key Laboratory of Crop Genetics and Breeding, Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050035, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

High oleic-acid peanuts are known for their pre-longed shelf-life and health benefit due to high content of oleic fatty acid. However, the allergenicity and allergenic protein profiles in Chinese high-oleic peanuts have yet to be studied. For this purpose, an Orbitrap Fusion mass spectrometry (MS)-based method that is feasible for identification of putative allergenic protein as well as semi-quantitation of five major allergen protein in three different Chinese high-oleic peanut cultivars (JH 13, JH 16 and JH 18) have been reported. Results show that three Chinese high-oleic acid peanut cultivars selected all contained highly allergenic proteins Ara h 1, Ara h 2, Ara h 3 and Ara h 6. The allergenic protein profiles of Chinese high-oleic acid peanut cultivars were very similar to that of conventional peanuts, but the allergenic protein subunits varied greatly among high-oleic peanuts. Additionally, a comprehensive peptide-filtering pipeline had been developed for identification of potential peptide markers in peanut allergen proteins. Through the peptide-filtering pipeline, three novel peptide markers, IVQIEAKPNTLVLPK, SSNPDIYNPQAGSLR and AQSENYEYLAFK surrogate to Ara h 1, Ara h 3 with high abundance, good MS response and highly reliability were identified, which can be used as candidate peptide markers for the detection of peanut allergens in different food matrices.

Keywords

AllergyHigh-oleic acid peanutPeptide-filtering pipelineNanoLC-Orbitrap mass spectrometry

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Food Science and Human Wellness
Volume 12 Issue 3,
May 2023
Pages 851-860
DOI: 10.1016/j.fshw.2022.09.026
Cite this article:
Yang X, Bai H, Yin L, et al. Evaluation of allergenic protein profiles in three Chinese high-oleic acid peanut cultivars using NanoLC-Orbitrap mass spectrometry. Food Science and Human Wellness, 2023, 12(3): 851-860. https://doi.org/10.1016/j.fshw.2022.09.026

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Received: 08 June 2021
Revised: 18 July 2021
Accepted: 29 August 2021
Published: 15 October 2022
© 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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