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Research Article | Open Access

A heteropolysaccharide from Rhodiola rosea L.: preparation, purification and anti-tumor activities in H22-bearing mice

Yaru Wu, Qing Wang, Huiping Liu( ), Lulu Niu, Mengyu Li, Qi Jia
State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

Numerous polysaccharides isolated from plants have been used to augment traditional drugs in the treatment of cancer. In order to explore the influence to hepatocellular carcinoma, a novel cold water-soluble polysaccharide was separated from Rhodiola rosea L. root (RLP) and then its structure and anti-cancer activities were tested. The chemical compositions and high performance gel permeation chromatography (HPGPC) results indicated that RLP was an acid heteropolysaccharide with the molecular weight of about 1.15×106 Da. Furthermore, ion chromatography (IC), Fourier transform infrared (FT-IR) and nuclear magnetic resoance (NMR) further indicated that RLP was main composed of →2,4)-α-Rha(1→, →5)-α-L-Araf-(1→, α-D-Glu, →6)-β-D-Galp-(1→, β-D-Man and →4)-α-GalpA-(1→. In vivo antitumor activities of RLP were carried out by using H22 tumor-bearing mice model. The results shown that RLP (100 and 300 mg/kg) could inhibit tumor growth of H22 cells from 23.59% to 45.52% and protect thymuses and spleen without damage. In addition, according to cell cycle, AV-FITC/PI and JC-1, RLP could induce dose-dependent apoptosis of H22 cells via S phase arrested which was through a mitochondrial related pathway. Our data indicated that RLP has a broader application prospect in anti-tumor preparations.

Keywords

PolysaccharideAntitumor activityRhodiola rosea L.Structure analysis

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Food Science and Human Wellness
Volume 12 Issue 2,
March 2023
Pages 536-545
DOI: 10.1016/j.fshw.2022.07.056
Cite this article:
Wu Y, Wang Q, Liu H, et al. A heteropolysaccharide from Rhodiola rosea L.: preparation, purification and anti-tumor activities in H22-bearing mice. Food Science and Human Wellness, 2023, 12(2): 536-545. https://doi.org/10.1016/j.fshw.2022.07.056

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Received: 17 December 2020
Revised: 01 March 2021
Accepted: 05 June 2021
Published: 07 September 2022
© 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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