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Research Article | Open Access

Fecal metabolomics reveals the positive effect of ethanol extract of propolis on T2DM mice

Chunmei Wanga, Huiting Zhaob, Kai Xuc, Yali Dud, Jinjia Liua, Jinfei Wanga, Yusuo Jianga( )
College of Animal Science, Shanxi Agricultural University, Taigu 030801, China
College of Life Sciences, Shanxi Agricultural University, Taigu 030801, China
Jilin Province Institute of Apicultural Science, Jilin 132108, China
College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

A large number of studies have shown that propolis has positive effects in the treatment of type 2 diabetes mellitus (T2DM). However, there are have only been a few reports that are based on an ultra-high performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UPLC-Q-TOF-MS) analysis of the fecal metabolomics of ethanol extract of propolis (EEP) in the treatment of T2DM. The present investigation was designed to screen potential biomarkers of T2DM by the metabonomic method and to explain the possible anti-diabetes mechanism of EEP according to the changes in the biomarkers. The results showed that EEP improved the body weight (BW) of T2DM mice, lowered blood sugar levels, and significantly restored blood biochemical indicators related to T2DM, such as fasting insulin (FINS), homeostasis model assessment of insulin resistance (HOMA-IR), aspartate transaminase (AST), and alanine aminotransferase (ALT). Liver pathology showed that EEP reversed liver damage caused by T2DM. Metabolomics data identified 27 potential biomarkers in fecal samples. EEP effectively regulated the dysfunction in the metabolic pathways of glycerophospholipids, sphingolipids, riboflavins, and sterol lipids caused by T2DM. In summary, our research results revealed positive effects of EEP in the treatment of T2DM and provided potential candidate markers for further research and in the clinical treatment of T2DM.

Keywords

Ethanol extract of propolisType 2 diabetes mellitusFecal metabolitesUPLC-Q-TOF-MSMetabolomics

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Food Science and Human Wellness
Volume 12 Issue 1,
January 2023
Pages 161-172
DOI: 10.1016/j.fshw.2022.07.034
Cite this article:
Wang C, Zhao H, Xu K, et al. Fecal metabolomics reveals the positive effect of ethanol extract of propolis on T2DM mice. Food Science and Human Wellness, 2023, 12(1): 161-172. https://doi.org/10.1016/j.fshw.2022.07.034

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Received: 04 February 2021
Revised: 27 February 2021
Accepted: 02 May 2021
Published: 09 August 2022
© 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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