Deep eutectic solvents and alkaline extraction of protein from seabuckthorn seed meal: a comparison study

Junjie Lin; Huan Xiang; Dongxiao Sun-Waterhouse; Chun Cui; Wei Wang

doi:10.1016/j.fshw.2022.03.019

Food Science and Human Wellness 2022, 11(4): 1028-1035 https://doi.org/10.1016/j.fshw.2022.03.019

Research Article |

Open Access | Issue | Published: 28 April 2022

Deep eutectic solvents and alkaline extraction of protein from seabuckthorn seed meal: a comparison study

Show Author's Information Hide Author's Information Junjie Lin^{^a}, Huan Xiang^{^a}, Dongxiao Sun-Waterhouse^{^a}, Chun Cui^{^a^,^b}(

), Wei Wang^{^a}

College of Food Science and Technology, South China University of Technology, Guangzhou 510640, China

Guangdong Wei-Wei Biotechnology Co., Ltd, Guangzhou 510640, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Keywords:

Amino acids, Deep eutectic solvent, Seabuckthorn seed meal, Protein extraction, Alkaline extraction and acid precipitation

Cite this article:

Lin J, Xiang H, Sun-Waterhouse D, et al. Deep eutectic solvents and alkaline extraction of protein from seabuckthorn seed meal: a comparison study. Food Science and Human Wellness, 2022, 11(4): 1028-1035. https://doi.org/10.1016/j.fshw.2022.03.019

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Abstract

Seabuckthorn seed meal (SSM) is a waste of oil extraction industry that rich in protein. In order to seek suitable protein extraction method, three different deep eutectic solvents (DESs) (including choline chloride-glycerol, choline chloride-oxalic acid and choline chloride-urea) were developed for extracting protein from SSM and compared with alkaline. Result indicated that alkaline could effectively extract 56.9% protein from SSM and its protein content was 73.1%, higher than DES at 31.0%‒41.4% and 64.3%‒67.5%, respectively. However, compared to alkali, DES led to a product with less β-sheet, more β-turn, more essential amino acids, higher total amino acid content, especially choline chloride-urea which extracted protein showing an integrated and similar protein weight distribution compared to SSM. Also, this protein extracted chloride-urea showed a highest digestibility in vitro (by pepsin) (54.2%). These results indicated that choline chloride-urea extraction is better than alkaline extraction for SSM.

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About this article

Deep eutectic solvents and alkaline extraction of protein from seabuckthorn seed meal: a comparison study

Show Author's information Hide Author's Information Junjie Lin^{^a}, Huan Xiang^{^a}, Dongxiao Sun-Waterhouse^{^a}, Chun Cui^{^a^,^b}(

), Wei Wang^{^a}

College of Food Science and Technology, South China University of Technology, Guangzhou 510640, China

Guangdong Wei-Wei Biotechnology Co., Ltd, Guangzhou 510640, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Abstract

Keywords: Amino acids, Deep eutectic solvent, Seabuckthorn seed meal, Protein extraction, Alkaline extraction and acid precipitation

References(44)

[1]

G. Suryakumar, A. Gupta, Medicinal and therapeutic potential of sea buckthorn (Hippophae rhamnoides L. ), J. Ethnopharmacol. 138 (2011) 268-278. https://doi.org/10.1016/j.jep.2011.09.024.

DOI Google Scholar

[2]

H. Yuan, F. Shi, L. Meng, et al., Effect of sea buckthorn protein on the intestinal microbial community in streptozotocin-induced diabetic mice, Int. J. Biol. Macromol. 107 (2018) 1168-1174. https://doi.org/10.1016/j.ijbiomac.2017.09.090.

DOI Google Scholar

[3]

H. Yuan, X. Zhu, W. Wang, et al., Hypoglycemic and anti-inflammatory effects of seabuckthorn seed protein in diabetic ICR mice, Food Funct. 7 (2016) 1610-1615. https://doi.org/10.1039/C5FO01600B.

DOI Google Scholar

[4]

H. Yuan, L. Meng, W. Wang, et al., The effects of sea buckthorn seed protein on glucose metabolism in streptozotocin-induced diabetic ICR mice, Pak. J. Pharm. Sci. 32 (5) (2019) 2011-2017.

Google Scholar

[5]

M. Cui, N. Tang, C. Chen, et al., Functional properties of seabuckthorn seed meal protein, China Oils and Fats (2012) 15.

Google Scholar

[6]

F. Hou, W. Ding, W. Qu, et al., Alkali solution extraction of rice residue protein isolates: influence of alkali concentration on protein functional, structural properties and lysinoalanine formation, Food Chem. 218 (2017) 207-215. https://doi.org/10.1016/j.foodchem.2016.09.064.

DOI Google Scholar

[7]

X. Mao, N. Guo, J. Sun, et al., Comprehensive utilization of shrimp waste based on biotechnological methods: a review, J. Clean. Prod. 143 (2017) 814-823. https://doi.org/10.1016/j.jclepro.2016.12.042.

DOI Google Scholar

[8]

R. Wahlström, K. Rommi, P. Willberg-Keyriläinen, et al., High yield protein extraction from Brewer's spent grain with novel carboxylate salturea aqueous deep eutectic solvents, Chemistry Select 2 (2017) 9355-9363. https://doi.org/10.1002/slct.2017014.

DOI Google Scholar

[9]

F. Qin, A.Z. Johansen, S.I. Mussatto, Evaluation of different pretreatment strategies for protein extraction from Brewer's spent grains, Ind. Crop. Prod. 125 (2018) 443-453. https://doi.org/10.1016/j.indcrop.2018.09.017.

DOI Google Scholar

[10]

Y. Marcus, Deep eutectic solvents in extraction and sorption technology, in: deep eutectic solvents, Springer, 2019 153-183. https://doi.org/10.1007/978-3-030-00608-2_5.

DOI

[11]

S.C. Cunha, J.O. Fernandes, Extraction techniques with deep eutectic solvents, TrAC Trends Anal. Chem. 105 (2018) 225-239. https://doi.org/10.1016/j.trac.2018.05.001.

DOI Google Scholar

[12]

Q. Zhang, K.D.O. Vigier, S. Royer, et al., Deep eutectic solvents: syntheses, properties and applications, Chem. Soc. Rev. 41 (2012) 7108-7146. https://doi.org/10.1039/C2CS35178A.

DOI Google Scholar

[13]

A. Grudniewska, E. M. de Melo, A. Chan, et al., Enhanced protein extraction from oilseed cakes using glycerol-choline chloride deep eutectic solvents: a biorefinery approach, ACS Sustain. Chem. Eng. 6 (2018) 15791-15800. https://doi.org/10.1021/acssuschemeng.8b04359.

DOI Google Scholar

[14]

C. Bai, Q. Wei, X. Ren, Selective extraction of collagen peptides with high purity from cod skins by deep eutectic solvents, ACS Sustain. Chem. Eng. 5 (2017) 7220-7227. https://doi.org/10.1021/acssuschemeng.7b01439.

DOI Google Scholar

[15]

G. A. Ruiz, W. Xiao, M. van Boekel, et al., Effect of extraction pH on heatinduced aggregation, gelation and microstructure of protein isolate from quinoa (Chenopodium quinoa Willd), Food Chem. 209 (2016) 203-210. https://doi.org/10.1016/j.foodchem.2016.04.052.

DOI Google Scholar

[16]

Y. Zhang, F. Zhou, M. Zhao, et al., Soy peptide aggregates formed during hydrolysis reduced protein extraction without decreasing their nutritional value, Food Func. 8 (2017) 4384-4395. https://doi.org/10.1039/C7FO00812K.

DOI Google Scholar

[17]

C. Wang, L. Jiang, D. Wei, et al., Effect of secondary structure determined by FTIR spectra on surface hydrophobicity of soybean protein isolate, Procedia Eng. 15 (2011) 4819-4827. https://doi.org/10.1016/j.proeng.2011.08.900.

DOI Google Scholar

[18]

B. Prandi, A. Faccini, F. Lambertini, et al., Food wastes from agrifood industry as possible sources of proteins: a detailed molecular view on the composition of the nitrogen fraction, amino acid profile and racemisation degree of 39 food waste streams, Food Chem. 286 (2019) 567-575. https://doi.org/10.1016/j.foodchem.2019.01.166.

DOI Google Scholar

[19]

P. Chakraborty, A. Mallik, N. Sarang, et al., A review on alternative plant protein sources available for future sustainable aqua feed production, Int. J. Chem. Stud. 7 (2019) 1399-1404.

Google Scholar

[20]

C. Basset, S. Kedidi, A. Barakat, Chemical-and solvent-free mechanophysical fractionation of biomass induced by tribo-electrostatic charging: separation of proteins and lignin, ACS Sustain. Chem. Eng. 4 (2016) 4166-4173. https://doi.org/10.1021/acssuschemeng.6b00667.

DOI Google Scholar

[21]

N. Xia, J.M. Wang, Q. Gong, et al., Characterization and in vitro digestibility of rice protein prepared by enzyme-assisted microfluidization: comparison to alkaline extraction, J. Cereal Sci. 56 (2012) 482-489. https://doi.org/10.1016/j.jcs.2012.06.008.

DOI Google Scholar

[22]

X. Li, J. Deng, S. Shen, et al., Antioxidant activities and functional properties of enzymatic protein hydrolysates from defatted Camellia oleifera seed cake, J. Food Sci. Tech. 52 (2015) 5681-5690. https://doi.org/10.1007/s13197-014-1693-z.

DOI Google Scholar

[23]

A.M. Ghribi, I.M. Gafsi, A. Sila, et al., Effects of enzymatic hydrolysis on conformational and functional properties of chickpea protein isolate. Food Chem. 187 (2015) 322-330. https://doi.org/10.1016/j.foodchem.2015.04.109.

DOI Google Scholar

[24]

J.Y. Yang, B. Peng, M. Wang, et al., Characteristics and emulsifying properties of two protein fractions derived from the emulsion formed during aqueous extraction of camellia oil, Food Hydrocolloid. 87 (2019) 644-652. https://doi.org/10.1016/j.foodhyd.2018.08.043.

DOI Google Scholar

[25]

A. Desai, T. Beibeia, M. Brennan, et al., Protein, amino acid, fatty acid composition, and in vitro digestibility of bread fortified with Oncorhynchus tschawytscha powder, Nutrients 10(12) (2018) 1923. https://doi.org/10.3390/nu10121923.

DOI Google Scholar

[26]

S. Bleakley, M. Hayes, Algal proteins: extraction, application, and challenges concerning production, Foods 6 (2017) 33. https://doi.org/10.3390/foods6050033.

DOI Google Scholar

[27]

Z. Zhang, Y. Wang, Y. Li, et al., Effect of alkali concentration on digestibility and absorption characteristics of rice residue protein isolates and lysinoalanine, Food Chem. 289 (2019) 609-615. https://doi.org/10.1016/j.foodchem.2019.03.085.

DOI Google Scholar

[28]

M. Carbonaro, P. Maselli, A. Nucara, Relationship between digestibility and secondary structure of raw and thermally treated legume proteins: a Fourier transform infrared (FT-IR) spectroscopic study, Amino acids, 43 (2012) 911-921. https://doi.org/10.1007/s00726-011-1151-4.

DOI Google Scholar

[29]

R. Arimboor, C. Arumughan, Sea buckthorn (Hippophae rhamnoides) proanthocyanidins inhibit in vitro enzymatic hydrolysis of protein, J. Food Sci. 76 (2011) T130-T137. https://doi.org/10.1111/j.1750-3841.2011.02238.x.

DOI Google Scholar

[30]

T. Bosiljkov, F. Dujmic, M. Cvjetko Bubalo, et al., Natural deep eutectic solvents and ultrasound-assisted extraction: green approaches for extraction of wine lees anthocyanins, Food Bioprod. Process. 102 (2017) 195-203. https://doi.org/10.1016/j.fbp.2016.12.005.

DOI Google Scholar

[31]

M.C. Bubalo, N. Curko, M. Tomasevic, et al., Green extraction of grape skin phenolics by using deep eutectic solvents, Food Chem. 200 (2016) 159-166. https://doi.org/10.1016/j.foodchem.2016.01.040.

DOI Google Scholar

[32]

S. Pradyawong, G. Qi, X.S. Sun, et al., Laccase/TEMPO-modified lignin improved soy-protein-based adhesives: adhesion performance and properties, Int. J. Adhes. Adhes. 91 (2019) 116-122. https://doi.org/10.1016/j.ijadhadh.2019.03.005.

DOI Google Scholar

[33]

X. Zhao, H. Liu, X. Zhang, et al., Comparison of structures of walnut protein fractions obtained through reverse micelles and alkaline extraction with isoelectric precipitation, Int. J. Biol. Macromol. 125 (2019) 1214-1220. https://doi.org/10.1016/j.ijbiomac.2018.09.095.

DOI Google Scholar

[34]

A.M.C. Marcelino, L.M. Gierasch, Roles of β-turns in protein folding: from peptide models to protein engineering, Biopolymers 89 (2008) 380-391. https://doi.org/10.1002/bip.20960.

DOI Google Scholar

[35]

A. Sivam, D. Sun-Waterhouse, C. Perera, et al., Exploring the interactions between blackcurrant polyphenols, pectin and wheat biopolymers in model breads; a FTIR and HPLC investigation, Food Chem. 131 (2012) 802-810. https://doi.org/10.1016/j.foodchem.2011.09.047.

DOI Google Scholar

[36]

N. Gharbi, M. Labbafi, Effect of processing on aggregation mechanism of egg white proteins, Food Chem. 252 (2018) 126-133. https://doi.org/10.1016/j.foodchem.2018.01.088.

DOI Google Scholar

[37]

J.B. Reeves Ⅲ, W.F. Schmidt, Solid-state ¹³C NMR analysis of forage and byproductderived fiber and lignin residues. Resolution of some discrepancies among chemical, infrared, and pyrolysis-gas chromatographymass spectroscopic analyses, J. Agr. Food Chem. 42 (1994) 1462-1468. https://doi.org/10.1021/jf00043a014.

DOI Google Scholar

[38]

A. Martínez-Richa, R.L. Silvestri (Ed.), Developments in Solid-State NMR Spectroscopy of Polymer Systems, IntechOpen, 2017. DOI:10.5772/intechopen.70116.

DOI

[39]

H. Kricheldorf, D. Müller, Secondary structure of peptides 16 th. Characterization of proteins by means of ¹³C NMR CP/MAS spectroscopy, Colloid Polym. Sci. 262 (1984) 856-861. https://doi.org/10.1007/BF01452215.

DOI Google Scholar

[40]

D.S. Wishart, B.D. Sykes, F.M. Richards, Relationship between nuclear magnetic resonance chemical shift and protein secondary structure, J. Mol. Bio. 222 (1991) 311-333. https://doi.org/10.1016/0022-2836(91)90214-Q.

DOI Google Scholar

[41]

C.M. Renard, M.C. Jarvis, A cross-polarization, magic-angle-spinning, ¹³C-nuclearmagnetic-resonance study of polysaccharides in sugar beet cell walls. Plant Physiol. 119 (1999) 1315-1322. https://doi.org/10.1104/pp.119.4.1315.

DOI Google Scholar

[42]

L. Pizzoferrato, P. Manzi, F. Bertocchi, et al., Solid-state ¹³C CP MAS NMR spectroscopy of mushrooms gives directly the ratio between proteins and polysaccharides, J. Agr. Food Chem. 48 (2000) 5484-5488. https://doi.org/10.1021/jf000448j.

DOI Google Scholar

[43]

X.J. Pan, Y. Sano, Comparison of acetic acid lignin with milled wood and alkaline lignins from wheat straw, Holzforschung 54 (2000) 61-65. https://doi.org/10.1515/HF.2000.009.

DOI Google Scholar

[44]

G.C. Xu, J.C. Ding, R.Z. Han, et al., Enhancing cellulose accessibility of corn stover by deep eutectic solvent pretreatment for butanol fermentation, Bioresource Technol. 203 (2016) 364-369. https://doi.org/10.1016/j.biortech.2015.11.002.

DOI Google Scholar

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Publication history

Received: 17 November 2020

Revised: 04 January 2021

Accepted: 03 February 2021

Published: 28 April 2022

Issue date: July 2022

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Acknowledgements

The authors are grateful for the financial support from the National Natural Science Foundation of China (No. 31201416) and Science and Technology Research Program of Guangdong Province (No. 2017A01010502).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).