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Food Science and Human Wellness 2022, 11(4): 806-814 https://doi.org/10.1016/j.fshw.2022.03.012
Research Article | Open Access | Issue | Published: 28 April 2022

Immunoregulatory polysaccharides from Apocynum venetum L. flowers stimulate phagocytosis and cytokine expression via activating the NF-κB/MAPK signaling pathways in RAW264.7 cells

Show Author's Information Honglin Wanga,b,1,  Changyang Maa,1,  Dongxiao Sun-Waterhousea,c,  Jinmei Wanga( ),  Geoffrey Ivan Neil Waterhousec( ),  Wenyi Kanga,d( )
National R&D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China
Functional Food Engineering Technology Research Center, Kaifeng 475004, China
School of Chemical Sciences, University of Auckland, Auckland 1142, New Zealand
Joint International Research Laboratory of Food & Medicine Resource Function, Kaifeng 475004, China

1 The authors contributed equally to this work.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

Keywords:
Apocynum venetum L. flowers, Immunomodulatory polysaccharide, RAW264.7 cells, NF-κB signaling pathway, MAPK signaling pathway
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Wang H, Ma C, Sun-Waterhouse D, et al. Immunoregulatory polysaccharides from Apocynum venetum L. flowers stimulate phagocytosis and cytokine expression via activating the NF-κB/MAPK signaling pathways in RAW264.7 cells. Food Science and Human Wellness, 2022, 11(4): 806-814. https://doi.org/10.1016/j.fshw.2022.03.012
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Abstract Full text About this article

Two immunomodulatory polysaccharides (Vp2a-Ⅱ and Vp3) were isolated and identified from Apocynum venetum L. flowers, and their innate immune-stimulating functions and working mechanisms were evaluated in RAW264.7 cells. Both the level of released nitric oxide (NO) and expression of inducible nitric oxide synthase (iNOS) mRNA were significantly enhanced in the RAW264.7 macrophages cells treated by Vp2a-Ⅱ and Vp3. Vp2a-Ⅱ (100–800 µg/mL) and Vp3 (400 µg/mL) could significantly increase the phagocytic activity of RAW264.7 cells and the secretion and mRNA expression of TNF-α and IL-6 in a concentration-dependent manner through affecting mitogen-activated protein kinase (MAPK) activity and nuclear factor κB (NF-κB) nuclear translocation. Vp2a-Ⅱ might activate the MAPK signaling pathways and induce the nuclear translocation of NF-κB p65, whilst Vp3 likely activated the NF-κB and MAPK signaling pathways without influencing the p38 MAPK route.


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Immunoregulatory polysaccharides from Apocynum venetum L. flowers stimulate phagocytosis and cytokine expression via activating the NF-κB/MAPK signaling pathways in RAW264.7 cells

Show Author's information Honglin Wanga,b,1, Changyang Maa,1, Dongxiao Sun-Waterhousea,c, Jinmei Wanga( ), Geoffrey Ivan Neil Waterhousec( ), Wenyi Kanga,d( )
National R&D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China
Functional Food Engineering Technology Research Center, Kaifeng 475004, China
School of Chemical Sciences, University of Auckland, Auckland 1142, New Zealand
Joint International Research Laboratory of Food & Medicine Resource Function, Kaifeng 475004, China

1 The authors contributed equally to this work.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

Abstract

Two immunomodulatory polysaccharides (Vp2a-Ⅱ and Vp3) were isolated and identified from Apocynum venetum L. flowers, and their innate immune-stimulating functions and working mechanisms were evaluated in RAW264.7 cells. Both the level of released nitric oxide (NO) and expression of inducible nitric oxide synthase (iNOS) mRNA were significantly enhanced in the RAW264.7 macrophages cells treated by Vp2a-Ⅱ and Vp3. Vp2a-Ⅱ (100–800 µg/mL) and Vp3 (400 µg/mL) could significantly increase the phagocytic activity of RAW264.7 cells and the secretion and mRNA expression of TNF-α and IL-6 in a concentration-dependent manner through affecting mitogen-activated protein kinase (MAPK) activity and nuclear factor κB (NF-κB) nuclear translocation. Vp2a-Ⅱ might activate the MAPK signaling pathways and induce the nuclear translocation of NF-κB p65, whilst Vp3 likely activated the NF-κB and MAPK signaling pathways without influencing the p38 MAPK route.

Keywords: Apocynum venetum L. flowers, Immunomodulatory polysaccharide, RAW264.7 cells, NF-κB signaling pathway, MAPK signaling pathway

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Received: 06 September 2020
Revised: 21 September 2020
Accepted: 18 October 2021
Published: 28 April 2022
Issue date: July 2022

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© 2022 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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This work was supported by Research on Precision Nutrition and Health Food, Department of Science and Technology of Henan Province (CXJD2021006).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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