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Food Science and Human Wellness 2022, 11(4): 795-805 https://doi.org/10.1016/j.fshw.2022.03.010
Research Article | Open Access | Issue | Published: 28 April 2022

GPP (composition of Ganoderma lucidum polysaccharides and Polyporus umbellatus polysaccharides) protects against DSS-induced murine colitis by enhancing immune function and regulating intestinal flora

Show Author's Information Liyuan Lia,b,1,  Yannan Guoa,1,  Qing Huanga,  Xiaojin Shia,  Qingqing Liuc,  Fang Wangd,  Qingfei Liua,  Kang Yud,  Zhao Wanga( )
MOE Key Laboratory of Protein Science, School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China
Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China
Tianjin Research Institute for Advanced Equipment, Tianjin 300300, China
Department of Clinical Nutrition and Department of Health Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China

1 The authors contributed equally to this work.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

Keywords:
Intestinal flora, Ganoderma lucidum polysaccharides, Polyporus umbellatus polysaccharides, Immune function, Colitis
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Li L, Guo Y, Huang Q, et al. GPP (composition of Ganoderma lucidum polysaccharides and Polyporus umbellatus polysaccharides) protects against DSS-induced murine colitis by enhancing immune function and regulating intestinal flora. Food Science and Human Wellness, 2022, 11(4): 795-805. https://doi.org/10.1016/j.fshw.2022.03.010
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Abstract Full text About this article

Previous study have demonstrated that a compound composed of water-soluable Ganoderma lucidum polysaccharides (GLP) and Polyporus umbellatus polysaccharides (PUP) in a ratio of 3:1 named GPP enhances innate immune function in mice through enhancing the function of macrophage cells and activity of natural killer (NK) cells. Here in our research, we further investigated the effect of GPP on the diversity and composition of intestinal flora, and explored its effect on colitis model mice. The immunoregulatory verification experiments of GPP were conducted in both normal and DSS-induced mice model. Our research showed that GPP increased the diversity of intestinal microorganisms in mice with the extension of administration time. Daily GPP intake attenuated DSS-induced colon injury, protected the splenic lymphocyte proliferation ability, enhanced the serum hemolysin synthesis, and increased peripheral phagocytes and NK cell activity in model mice. Comparisons of the predominant gene pathways of the bacterial microbiota showed that DNA repair and recombination, base mismatch repair pathways was stronger in GPP-treatment group than in control group, indicating the possible molecular mechanisms of immune function regulation. Our study showed that GPP regulated immune function in both health and colitis model, and had a positive effect on maintaining intestinal flora homeostasis.


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About this article

GPP (composition of Ganoderma lucidum polysaccharides and Polyporus umbellatus polysaccharides) protects against DSS-induced murine colitis by enhancing immune function and regulating intestinal flora

Show Author's information Liyuan Lia,b,1, Yannan Guoa,1, Qing Huanga, Xiaojin Shia, Qingqing Liuc, Fang Wangd, Qingfei Liua, Kang Yud, Zhao Wanga( )
MOE Key Laboratory of Protein Science, School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China
Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China
Tianjin Research Institute for Advanced Equipment, Tianjin 300300, China
Department of Clinical Nutrition and Department of Health Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China

1 The authors contributed equally to this work.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

Abstract

Previous study have demonstrated that a compound composed of water-soluable Ganoderma lucidum polysaccharides (GLP) and Polyporus umbellatus polysaccharides (PUP) in a ratio of 3:1 named GPP enhances innate immune function in mice through enhancing the function of macrophage cells and activity of natural killer (NK) cells. Here in our research, we further investigated the effect of GPP on the diversity and composition of intestinal flora, and explored its effect on colitis model mice. The immunoregulatory verification experiments of GPP were conducted in both normal and DSS-induced mice model. Our research showed that GPP increased the diversity of intestinal microorganisms in mice with the extension of administration time. Daily GPP intake attenuated DSS-induced colon injury, protected the splenic lymphocyte proliferation ability, enhanced the serum hemolysin synthesis, and increased peripheral phagocytes and NK cell activity in model mice. Comparisons of the predominant gene pathways of the bacterial microbiota showed that DNA repair and recombination, base mismatch repair pathways was stronger in GPP-treatment group than in control group, indicating the possible molecular mechanisms of immune function regulation. Our study showed that GPP regulated immune function in both health and colitis model, and had a positive effect on maintaining intestinal flora homeostasis.

Keywords: Intestinal flora, Ganoderma lucidum polysaccharides, Polyporus umbellatus polysaccharides, Immune function, Colitis

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Publication history

Received: 14 January 2021
Revised: 12 February 2021
Accepted: 18 March 2021
Published: 28 April 2022
Issue date: July 2022

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© 2022 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Acknowledgements

This study was financially supported by grants from the National Key R&D Program of China (2018YFD0400204), the National Natural Science Foundation of China (81974503, 81871095), and the Key International S&T Cooperation Program of China (2016YFE113700).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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