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To date, in vivo investigations of polysaccharide's pharmacokinetics are significantly restricted by the difficulty in their detection. This study was conducted to establish the quantitative determination of Lycium barbarum polysaccharides (LBPs) based on fluorescein isothiocyanate (FITC) pre-labeling and to investigate their tissue distribution in rat. We obtained the calibration curves linear over the range of 0–25µg/mL in rat tissue samples with correlation coefficients greater than 0.99. The inter-day and intra-day precisions (RSD, %) were within 15%, and the relative recovery ranged 95.2%–102.4%, with RSD range 1.48%–9.58%, indicating that this experiment was suitable for the determination of LBPs. The fluorescence intensity was measured after 24 h storage at room temperature, 3 times of freeze-cycle and cryopreservation at –20 ℃ for 15 day, these results indicated that the stability of the samples was good. LBP-FITC was mainly absorbed by the small intestine and stomach, and mainly excreted in the urine through the kidney; this distinct difference in the tissue distribution of LBPs could be attributed to the size of these LBPs in relation to the pore sizes of the vascular beds in the kidney and liver. Results showed in this study enable us to comprehensively understand the biological effects of LBPs following its oral ingestion.


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Tissue distribution of Lycium barbarum polysaccharides in rat tissue by fluorescein isothiocyanate labeling

Show Author's information Chao Yang1Hui Xia1Huali TangLigang YangGuiju Sun( )
Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, and Department of Nutrition and Food hygiene, School of Public Health, Southeast University, China-DRIs Expert Committee on Other Food Substances, Nanjing 210009, China

1 The authors contributed equally to this article.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

Abstract

To date, in vivo investigations of polysaccharide's pharmacokinetics are significantly restricted by the difficulty in their detection. This study was conducted to establish the quantitative determination of Lycium barbarum polysaccharides (LBPs) based on fluorescein isothiocyanate (FITC) pre-labeling and to investigate their tissue distribution in rat. We obtained the calibration curves linear over the range of 0–25µg/mL in rat tissue samples with correlation coefficients greater than 0.99. The inter-day and intra-day precisions (RSD, %) were within 15%, and the relative recovery ranged 95.2%–102.4%, with RSD range 1.48%–9.58%, indicating that this experiment was suitable for the determination of LBPs. The fluorescence intensity was measured after 24 h storage at room temperature, 3 times of freeze-cycle and cryopreservation at –20 ℃ for 15 day, these results indicated that the stability of the samples was good. LBP-FITC was mainly absorbed by the small intestine and stomach, and mainly excreted in the urine through the kidney; this distinct difference in the tissue distribution of LBPs could be attributed to the size of these LBPs in relation to the pore sizes of the vascular beds in the kidney and liver. Results showed in this study enable us to comprehensively understand the biological effects of LBPs following its oral ingestion.

Keywords: Lycium barbarum polysaccharides, Fluorescein isothiocyanate, Tissue distribution

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Received: 05 October 2020
Revised: 19 February 2021
Accepted: 18 January 2021
Published: 28 April 2022
Issue date: July 2022

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© 2022 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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Acknowledgement

The authors also thank the support from the National Key Research and Development Program of China (No. 2016YFD400604-02), the National Natural Science Foundation of China (No. 82073551, 82003457, 81273069), the Postgraduate Research & Practice Innovation Program of Jiangsu Province (No. KYCX19_0121), the Scientific Research Foundation of Graduate School of Southeast University (No. YBPY1944), the Fundamental Research Funds for the Central Universities (No. 2242020R10006), and CNS Research Fund for DRI.

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