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Food Science and Human Wellness 2022, 11(4): 771-780 https://doi.org/10.1016/j.fshw.2022.03.003
Research Article | Open Access | Issue | Published: 28 April 2022

Simultaneous quantification of 18 bioactive constituents in Ziziphus jujuba fruits by HPLC coupled with a chemometric method

Show Author's Information Tianyi Wanga,1,  Sen Guoa,b,1,  Xiameng Rena,  Junfeng Duc,  Lu Baib,  Xueqin Cuib,  Chi-Tang Hod( ),  Naisheng Baia( )
College of Food Science and Technology, Northwest University, Xi'an 710069, China
College of Chemical Engineering, Northwest University, 229 Taibai North Road, Xi'an 710069, China
Shaanxi Family Forestry Bureau, Shaanxi Jiaxian Development and Reform and Science and Technology Bureau, Yulin 719200, China
Department of Food Science, Rutgers University, New Brunswick 08901, USA

1 The authors contributed equally to this article.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

Keywords:
Quantitative analysis, Ziziphus jujuba, HPLC-DAD-MS, Hierarchical cluster analysis, HepG2 cells
Cite this article:
Wang T, Guo S, Ren X, et al. Simultaneous quantification of 18 bioactive constituents in Ziziphus jujuba fruits by HPLC coupled with a chemometric method. Food Science and Human Wellness, 2022, 11(4): 771-780. https://doi.org/10.1016/j.fshw.2022.03.003
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Abstract Full text About this article

The fruit of Ziziphus jujuba Mill., known as Hongzao (or Hong-Zao) in Chinese and cultivated in China for more than 4000 years, has shown to have hepatoprotective property. In previous study, we have isolated and identified 27 known compounds from Z. jujuba fruits, which demonstrated anti-tumor activity. In this study, a high-performance liquid chromatography-diode-array detection-mass spectrometry (HPLC-DAD-MS) method was successfully applied to the simultaneous characterization and quantitation of 18 constituents in 28 Z. jujuba samples, comprised of 12 cultivars from different regions in China, by comparing their HPLC retention times, MS spectra, UV spectra, and NMR data with those of reference compounds. The quantitative method was validated with excellent linearity (R2 > 0.999 1), preferable intra- and inter-day precisions (RSD < 2.78%), and good recoveries (94.96%–102.65%). The content variation of 18 compounds was analyzed by a chemometric method (hierarchical cluster analysis). In addition, these constituents showed protection against carbon tetrachloride (CCl4) intoxicated HepG2 cell lines by decreasing lactic dehydrogenase (LDH) levels. Results in this study illustrated that the content of all 18 compounds examined has significant difference and variation among cultivars and extracts. The proposed method can serve as a prerequisite for quality control of bioactive compounds in Z. jujuba products.


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About this article

Simultaneous quantification of 18 bioactive constituents in Ziziphus jujuba fruits by HPLC coupled with a chemometric method

Show Author's information Tianyi Wanga,1, Sen Guoa,b,1, Xiameng Rena, Junfeng Duc, Lu Baib, Xueqin Cuib, Chi-Tang Hod( ), Naisheng Baia( )
College of Food Science and Technology, Northwest University, Xi'an 710069, China
College of Chemical Engineering, Northwest University, 229 Taibai North Road, Xi'an 710069, China
Shaanxi Family Forestry Bureau, Shaanxi Jiaxian Development and Reform and Science and Technology Bureau, Yulin 719200, China
Department of Food Science, Rutgers University, New Brunswick 08901, USA

1 The authors contributed equally to this article.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

Abstract

The fruit of Ziziphus jujuba Mill., known as Hongzao (or Hong-Zao) in Chinese and cultivated in China for more than 4000 years, has shown to have hepatoprotective property. In previous study, we have isolated and identified 27 known compounds from Z. jujuba fruits, which demonstrated anti-tumor activity. In this study, a high-performance liquid chromatography-diode-array detection-mass spectrometry (HPLC-DAD-MS) method was successfully applied to the simultaneous characterization and quantitation of 18 constituents in 28 Z. jujuba samples, comprised of 12 cultivars from different regions in China, by comparing their HPLC retention times, MS spectra, UV spectra, and NMR data with those of reference compounds. The quantitative method was validated with excellent linearity (R2 > 0.999 1), preferable intra- and inter-day precisions (RSD < 2.78%), and good recoveries (94.96%–102.65%). The content variation of 18 compounds was analyzed by a chemometric method (hierarchical cluster analysis). In addition, these constituents showed protection against carbon tetrachloride (CCl4) intoxicated HepG2 cell lines by decreasing lactic dehydrogenase (LDH) levels. Results in this study illustrated that the content of all 18 compounds examined has significant difference and variation among cultivars and extracts. The proposed method can serve as a prerequisite for quality control of bioactive compounds in Z. jujuba products.

Keywords: Quantitative analysis, Ziziphus jujuba, HPLC-DAD-MS, Hierarchical cluster analysis, HepG2 cells

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Publication history

Received: 16 August 2020
Revised: 26 September 2021
Accepted: 03 December 2021
Published: 28 April 2022
Issue date: July 2022

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© 2022 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Acknowledgements

Acknowledgment

This study was supported by a grant from Desert Control Research Institute of Shaanxi Province (No. 203130012) and National Natural Science Foundation of China (No. 31570348).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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