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Food Science and Human Wellness 2022, 11(3): 686-696 https://doi.org/10.1016/j.fshw.2021.12.026
Research Article | Open Access | Issue | Published: 04 February 2022

Fungi with potential probiotic properties isolated from Fuzhuan brick tea

Show Author's Information Xin Wanga,b,  Yuanyuan Cuia,b,  Chaowei Sanga,  Bin Wanga,  Yirong Yuana,  Lin Liua,b,  Yahong Yuana,b( ),  Tianli Yuea,b,c( )
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
Laboratory of Quality & Safety Risk Assessment for Agro-products (YangLing), Ministry of Agriculture, Yangling 712100, China
College of Food Science and Technology, Northwest University, Xi'an 710069, China
Keywords:
Fungi, Fuzhuan brick tea, Candidate probiotics, Probiotic properties, Bacteriostatic activity, Adhesion capacity
Cite this article:
Wang X, Cui Y, Sang C, et al. Fungi with potential probiotic properties isolated from Fuzhuan brick tea. Food Science and Human Wellness, 2022, 11(3): 686-696. https://doi.org/10.1016/j.fshw.2021.12.026
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Abstract Full text About this article

Several of the fungal species associated with Fuzhuan brick tea (FBT) are considered as potential probiotics, but few studies have investigated the probiotic properties of these fungi. Here, we isolated 18 fungal strains from two types of FBT and identified these strains based on internal transcribed spacer (ITS) fragment sequence similarity to reference strains (sequence similarity > 98%). Of the 18 strains, 10 tolerated simulated human digestive conditions for sufficient periods in vitro: pH 2-3, 0.3%-0.5% (m/V) bile salts, and artificial gastrointestinal juices. We then measured the antimicrobial activity of the remaining 10 strains against 5 enteropathogenic bacteria and tested the bacteriostatic effects of the thalli and fermentation broth extracts. Of the 6 strains with strong bacteriostatic effects, we eliminated Eurotium cristatum S-9 due to its low hydrophobicity of (26.12 ± 0.35)%. Finally, 2 exhibited good adhesion abilities to human cells (> 100%). Notably, 2 strains can survive in vivo, because they can be isolated from C57BL/6 mice feces. Thus, 2 strains, Aspergillus cristatus H-1 and A. cristatus H-5, are herein identified as promising candidate probiotic strains. It may be put forward a novel research focus on evaluating potential probiotic fungi from FBT.


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About this article

Fungi with potential probiotic properties isolated from Fuzhuan brick tea

Show Author's information Xin Wanga,b, Yuanyuan Cuia,b, Chaowei Sanga, Bin Wanga, Yirong Yuana, Lin Liua,b, Yahong Yuana,b( ), Tianli Yuea,b,c( )
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
Laboratory of Quality & Safety Risk Assessment for Agro-products (YangLing), Ministry of Agriculture, Yangling 712100, China
College of Food Science and Technology, Northwest University, Xi'an 710069, China

Abstract

Several of the fungal species associated with Fuzhuan brick tea (FBT) are considered as potential probiotics, but few studies have investigated the probiotic properties of these fungi. Here, we isolated 18 fungal strains from two types of FBT and identified these strains based on internal transcribed spacer (ITS) fragment sequence similarity to reference strains (sequence similarity > 98%). Of the 18 strains, 10 tolerated simulated human digestive conditions for sufficient periods in vitro: pH 2-3, 0.3%-0.5% (m/V) bile salts, and artificial gastrointestinal juices. We then measured the antimicrobial activity of the remaining 10 strains against 5 enteropathogenic bacteria and tested the bacteriostatic effects of the thalli and fermentation broth extracts. Of the 6 strains with strong bacteriostatic effects, we eliminated Eurotium cristatum S-9 due to its low hydrophobicity of (26.12 ± 0.35)%. Finally, 2 exhibited good adhesion abilities to human cells (> 100%). Notably, 2 strains can survive in vivo, because they can be isolated from C57BL/6 mice feces. Thus, 2 strains, Aspergillus cristatus H-1 and A. cristatus H-5, are herein identified as promising candidate probiotic strains. It may be put forward a novel research focus on evaluating potential probiotic fungi from FBT.

Keywords: Fungi, Fuzhuan brick tea, Candidate probiotics, Probiotic properties, Bacteriostatic activity, Adhesion capacity

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Received: 18 May 2021
Revised: 01 September 2021
Accepted: 05 September 2021
Published: 04 February 2022
Issue date: May 2022

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© 2022 Beijing Academy of Food Sciences.

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This research was supported by the Shaanxi Special Project of China (2018ZDXM-NY-084).

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