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Research Article | Open Access

Dose-dependent effects of apple pectin on alleviating high fat-induced obesity modulated by gut microbiota and SCFAs

Yuanyuan Zhaoa, Jinfeng Bia ( ), Jianyong Yia( ), Jian Penga,b, Qiancheng Maa,c
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS)/Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, China
Sericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China
School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

Recently, pectin has been used as an alternative for commercial prebiotic products to modulate gut microbiota. In this study, the standard diet (Chow group) and high fat diet containing 0% (HF group), 1%, 3%, 5%, 8% or 12% (m/m) apple pectin (HF-P group) were offered ad libitum for 8 weeks to 4-week C57BL/6J mice (n=10/group). Results showed that body weight gain (R2=–0.990), subcutaneous adipose accumulation (R2=–0.930), serum triglyceride elevation (R2=–0.879), acetate (R2=0.955) and propionate concentration reduction (R2=0.985) were suppressed by pectin from 1% to 12%, indicating dose response to pectin consumption. Moreover, abundance of obesity-related bacteria Coriobacteriaceae, and pectin-degradating bacteria, Faecalibaculum, Ruminococcus were significantly increased with pectin dosage increased, and the R2 were 0.936, 0.963 and 0.937, respectively. Besides, attenuations for colonic injury and liver steatosis were observed after adding 5% and 8% pectin, respectively. This study confirmed the dose-sensitive manner of pectin to alleviate HF-induced obesity by modulating gut microbiota and enhancing short-chain fatty acids production.

Keywords

PectinDosageObesityGut microbiotaShort-chain fatty acid

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Food Science and Human Wellness
Volume 11 Issue 1,
January 2022
Pages 143-154
DOI: 10.1016/j.fshw.2021.07.015
Cite this article:
Zhao Y, Bi J, Yi J, et al. Dose-dependent effects of apple pectin on alleviating high fat-induced obesity modulated by gut microbiota and SCFAs. Food Science and Human Wellness, 2022, 11(1): 143-154. https://doi.org/10.1016/j.fshw.2021.07.015

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Received: 30 November 2020
Revised: 02 January 2021
Accepted: 03 January 2021
Published: 11 September 2021
© 2021 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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