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Research Article | Open Access

Structural characterization of oligosaccharide from Spirulina platensis and its effect on the faecal microbiota in vitro

Bingna Caia,b,1, Xiangxi Yic,1, Qian Hanc, Jianyu Pana,d, Hua Chena,b, Huili Suna, Peng Wana,d( )
Key Laboratory of Tropical Marine Bio-Resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301 Guangdong, China
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, 511458 Guangdong, China
School of Pharmacy, Guangxi University of Chinese Medicine, Nanning, 530200 Guangxi, China
Innovation Academy of South China Sea Ecology and Environmental Engineering (ISEE), Chinese Academy of Sciences, Guangzhou, 510000 Guangdong, China

1 These authors contributed equally to this work.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

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Abstract

In the present study, an oligosaccharide SPO-1 from Spirulina platensis was prepared by glycosidase from a marine bacterium. The prebiotic activity of SPO-1 on the growth of Lactobacillus paracasei and Bifidobacterium animalis, and its effect on human gut microbiota were examined in vitro. The molecular weight of the tetrasaccharide SPO-1 was 650.2 Da, and it was mainly composed of glucose with α-type glycosidic linkages. The prebiotic activity score of SPO-1 was the highest for the growth of probiotic strains L. paracasei and B. animalis. Furthermore, as fermentation proceeded, SPO-1 was gradually degraded and utilized by intestinal bacteria. The results showed that after treatment with SPO-1, carbohydrate consumption and short-chain fatty acids levels were increased, especially those of i-butyric and i-valeric acids. Moreover, SPO-1 significantly promoted the abundance, diversity and composition of gut microbiota, especially stimulating the growth of Bacteroides, Escherichia-Shigella and Megamonas. In addition, the change in intestinal microbiota function predicted by phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) after treatment with SPO-1 is mainly related to the terms "carbohydrate metabolism" and "amino acid metabolism". These results suggest that SPO-1 is a potential oligosaccharide in regulation of intestinal microbiota.

Keywords

OligosaccharideSpirulina platensisGut microbiotaIn vitro fermentation

References

[1]

Q.S. Shang, H. Jiang, C. Cai, et al., Gut microbiota fermentation of marine polysaccharides and its effects on intestinal ecology: an overview, Carbohyd. Polym. 179 (2018) 173-185. http://dx.doi.org/10.1016/j.carbpol.2017.09.059.
Crossref Google Scholar
[2]

R. Valcheva, L.A. Dieleman, Prebiotics: definition and protective mechanisms, Best Pract. Res. Cl. Ga. 30 (2016) 27-37. http://dx.doi.org/10.1016/j.bpg.2016.02.008.
Crossref Google Scholar
[3]

M.P.L. Guarino, A. Altomare, S. Emerenziani, et al., Mechanisms of action of prebiotics and their effects on gastro-intestinal disorders in adults, Nutrients 12 (2020) 1037. https://doi.org/10.3390/nu12041037.
Crossref Google Scholar
[4]

F.J. Moreno, N. Corzo, A. Montilla, et al., Current state and latest advances in the concept production and functionality of prebiotic oligosaccharides, Curr. Opin. Food Sci. 13 (2017) 50-55. http://dx.doi.org/10.1016/j.cofs.2017.02.009.
Crossref Google Scholar
[5]

R.R.R. Sardari, E.N. Karlsson, Marine poly- and oligosaccharides as prebiotics, J. Agr. Food Chem. 66(2018) 11544-11549. http://dx.doi.org/10.1021/acs.jafc.8b04418.
Crossref Google Scholar
[6]

A. Lopez-Santamarina, J.M. Miranda, A.D. Mondragon, et al., Potential use of marine seaweeds as prebiotics: a review, Molecules 25 (2020) 1004. http://dx.doi.org/10.3390/molecules25041004.
Crossref Google Scholar
[7]

M. Asadpoor, C. Peeters, P.A.J. Henricks, et al., Anti-pathogenic functions of non-digestible oligosaccharides in vitro, Nutrients 12 (2020) 1789. https://doi.org/10.3390/nu12061789.
Crossref Google Scholar
[8]

X. Zhang, J.J. Aweya, Z.X. Huang, et al., In vitro fermentation of Gracilaria lemaneiformis sulfated polysaccharides and its agaro-oligosaccharides by human faecal inocula and its impact on microbiota, Carbohyd. Polym. 234 (2020) 867-871. http://dx.doi.org/10.1016/j.carbpol.2020.115894.
Crossref Google Scholar
[9]

Y. Wang, F. Han, B. Hu, et al., In vivo prebiotic properties of alginate oligosaccharides prepared through enzymatic hydrolysis of alginate, Nutr. Res. 26 (2006) 597-603. http://dx.doi.org/10.1016/j.carbpol.2020.115894.
Crossref Google Scholar
[10]

J. Liu, S.Q. Yang, X.T. Li, et al., Alginate oligosaccharides: production, biological activities, and potential applications, Compr. Rev. Food Sci. F. 18 (2019) 1859-1881. http://dx.doi.org/10.1111/1541-4337.12494.
Crossref Google Scholar
[11]

D.D. Gurpilhares, L.P. Cinelli, N.K. Simas, et al., Marine prebiotics: polysaccharides and oligosaccharides obtained by using microbial enzymes, Food Chem. 280 (2019) 175-186. http://dx.doi.org/10.1016/j.foodchem.2018.12.023.
Crossref Google Scholar
[12]

M.F.D. Raposo, A.M.M.B. de Morais, R.M.S.C. de Morails, Emergent sources of prebiotics: seaweeds and microalgae, Mar. Drugs 14 (2016) 27. https://doi.org/10.3390/md14020027.
Crossref Google Scholar
[13]

Q.H. Wu, L. Liu, A. Miron, et al., The antioxidant, immunomodulatory, and anti-inflammatory activities of Spirulina: an overview, Arch. Toxicol. 90 (2016) 1817-1840. https://doi.org/10.1007/s00204-016-1744-5.
Crossref Google Scholar
[14]

F.Y.F. Hernandez, S. Khandual, I.G.R. Lopez, Cytotoxic effect of Spirulina platensis extracts on human acute leukemia Kasumi-1 and chronic myelogenous leukemia K-562 cell lines, Asian Pac. J. Trop. Bio. 7 (2017) 14-19. https://doi.org/10.1016/j.apjtb.2016.10.011.
Crossref Google Scholar
[15]

P. Rajasekar, S. Palanisamy, R. Anjali, et al., Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: in vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance, Int. J. Biol. Macromol. 141 (2019) 809-821. http://dx.doi.org/10.1016/j.ijbiomac.2019.09.024.
Crossref Google Scholar
[16]

A. Kulshreshtha, J.A. Zacharia, U. Jarouliya, et al., Spirulina in health care management, Curr. Pharm. Biotechno. 9 (2008) 400-405. http://dx.doi.org/10.2174/138920108785915111.
Crossref Google Scholar
[17]

H.P. Chen, F. Zeng, S.M. Li, et al., Spirulina active substance mediated gut microbes improve lipid metabolism in high-fat diet fed rats, J. Funct. Foods 59 (2019) 215-222. http://dx.doi.org/10.1016/j.jff.2019.04.049.
Crossref Google Scholar
[18]

H.T. Ma, H.Y. Xiong, X.L. Zhu, et al., Polysaccharide from Spirulina platensis ameliorates diphenoxylate-induced constipation symptoms in mice, Int. J. Biol. Macromol. 133 (2019) 1090-1101. https://doi.org/10.1016/j.ijbiomac.2019.04.209.
Crossref Google Scholar
[19]

F. Kurb, V. Samavati, Water soluble polysaccharides from Spirulina platensis: extraction and in vitro anti-cancer activity, Int. J. Biol. Macromol. 74 (2015) 498-506. https://doi.org/10.1016/j.ijbiomac.2015.01.005.
Crossref Google Scholar
[20]

J. Huebner, R.L. Wehling, R.W. Hutkins, Functional activity of commercial prebiotics, Int. Dairy J. 17 (2007) 770-775. http://dx.doi.org/10.1016/j.idairyj.2006.10.006.
Crossref Google Scholar
[21]

S. Affes, I. Aranaz, M. Hamdi, et al., Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan, Int. J. Biol. Macromol. 139 (2019) 558-569. http://dx.doi.org/10.1016/j.ijbiomac.2019.07.116.
Crossref Google Scholar
[22]

T. Di, G.J. Chen, Y. Sun, et al., In vitro digestion by saliva, simulated gastric and small intestinal juices and fermentation by human faecal microbiota of sulfated polysaccharides from Gracilaria rubra, J. Funct. Foods 40 (2018) 18-27. http://dx.doi.org/10.1016/j.jff.2017.10.040.
Crossref Google Scholar
[23]

F. Bianchi, M. Dall'Asta, D. Del Rio, et al., Development of a headspace solid-phase microextraction gas chromatography-mass spectrometric method for the determination of short-chain fatty acids from intestinal fermentation, Food Chem. 129 (2011) 200-205. http://dx.doi.org/10.1016/j.foodchem.2011.04.022.
Crossref Google Scholar
[24]

H.S. Wang, P.F. Ren, L. Mang, et al., In vitro fermentation of novel microwave-synthesized non-digestible oligosaccharides and their impact on the composition and metabolites of human gut microbiota, J. Funct. Foods 55 (2019) 156-166. http://dx.doi.org/10.1016/j.jff.2019.02.030.
Crossref Google Scholar
[25]

Q. Wang, G.M. Garrity, J.M. Tiedje, et al., Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy, Appl. Environ. Microb. 73 (2007) 5261−5267. http://doi.org/10.1128/AEM.00062-07.
Crossref Google Scholar
[26]

M. Kanehisa, S. Goto, Y. Sato, et al., KEGG for integration and interpretation of large-scale molecular data sets, Nucleic Acids Res. 40 (2012) D109−D114, http://dx.doi.org/10.1093/nar/gkr988.
Crossref Google Scholar
[27]

C.S. Wang, D.W. Zhang, M.L. Zhang, et al., Structural characterization of a novel oligosaccharide from Achyranthes bidentata and its anti-osteoporosis activities, Ind. Crop Prod. 108 (2017) 458−469, http://dx.doi.org/10.1016/j.indcrop.2017.07.018.
Crossref Google Scholar
[28]

H.Q. Sun, W.W. Song, L.J. Zhang, et al., Structural characterization and inhibition on α-glucosidase of a novel oligosaccharide from barley malt, J. Cereal. Sci. 28 (2018) 82-93. http://dx.doi.org/10.1016/j.jcs.2018.05.009.
Crossref Google Scholar
[29]

C. Altona, C.A.G. Haasnoot, Prediction of anti and gauche vicinal proton-proton coupling constants in carbohydrates: a simple additivity rule for pyranose rings, Org. Magn. Reson. 13 (1980) 417-429. http://doi.org/10.1002/mrc.12701306061980.
Crossref Google Scholar
[30]

W.T. Tian, X.W. Zhang, H.P. Liu, et al., Structural characterization of an acid polysaccharide from Pinellia ternate and its induction effect on apoptosis of Hep G2 cells, Int. J. Biol. Macromol. 153 (2020) 451-460. https://doi.org/10.1016/j.ijbiomac.2020.02.219.
Crossref Google Scholar
[31]

H.D. Holscher, Dietary fiber and prebiotics and the gastrointestinal microbiota, Gut Microbes 8 (2017) 172-184. http://dx.doi.org/10.1080/19490976.2017.1290756.
Crossref Google Scholar
[32]

P.P. Jutur, A.A. Nesamma, K.M. Shaikh, Algae-derived marine oligosaccharides and their biological applications, Front. Mar. Sci. 3 (2016) NUSP83. http://dx.doi.org/10.3389/fmars.2016.00083.
Crossref Google Scholar
[33]

K. Gerasimidis, K. Bryden, X.F. Chen, et al., The impact of food additives, artificial sweeteners and domestic hygiene products on the human gut microbiome and its fibre fermentation capacity, Eur. J. Nutr. 59 (2019) 3213-3230. http://dx.doi.org/10.1007/s00394-019-02161-8.
Crossref Google Scholar
[34]

J.J. Mou, Q. Li, W.W. Shi, et al., Chain conformation, physicochemical properties of fucosylated chondroitin sulfate from sea cucumber Stichopus chloronotus and its in vitro fermentation by human gut microbiota, Carbohyd. Polym. 228 (2020) UNSP115359. https://doi.org/10.1016/j.carbpol.2019.115359.
Crossref Google Scholar
[35]

S.A. Badi, A. Moshiri, F.E. Marvasti, et al., Extraction and evaluation of outer membrane vesicles from two important gut microbiota members, Bacteroids fragilis and Bacteroides thetaiotaomicron, Cell J. 22 (2020) 344−349, http://doi.org/10.22074/cellj.2020.6499.
Crossref Google Scholar
[36]

K.E. Deering, A. Devine, T.A. Q'Sullivan, et al., Characterizing the composition of the pediatric gut microbiome: a systematic review, Nutrients 12 (2020) 16. http://dx.doi.org/10.3390/nu12010016.
Crossref Google Scholar
[37]

M.E. Sanders, D.J. Merenstein, G. Reid, et al., Probiotics and prebiotics in intestinal health and disease: from biology to the clinic, Nat. Rev. Gastro. Hepat. 16 (2019) 605-616. http://dx.doi.org/10.1038/s41575-019-0173-3.
Crossref Google Scholar
[38]

Y. Cai, J. Folkerts, G. Folkerts, et al., Microbiota-dependent and independent effects of dietary fibre on human health, Brit. J. Pharmacol. 177 (2019) 1363-1381. http://dx.doi.org/10.1111/bph.14871.
Crossref Google Scholar
[39]

S. O'Connor, S. Chouinard-Castonguay, C. Gagnon et al., Prebiotics in the management of components of the metabolic syndrome, Maturitas 104 (2017) 11-18. http://dx.doi.org/10.1016/j.maturitas.2017.07.005.
Crossref Google Scholar
Food Science and Human Wellness
Volume 11 Issue 1,
January 2022
Pages 109-118
DOI: 10.1016/j.fshw.2021.07.012
Cite this article:
Cai B, Yi X, Han Q, et al. Structural characterization of oligosaccharide from Spirulina platensis and its effect on the faecal microbiota in vitro. Food Science and Human Wellness, 2022, 11(1): 109-118. https://doi.org/10.1016/j.fshw.2021.07.012

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Received: 22 July 2020
Revised: 24 September 2020
Accepted: 08 October 2020
Published: 11 September 2021
© 2021 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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