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Food Science and Human Wellness 2022, 11(1): 68-73 https://doi.org/10.1016/j.fshw.2021.07.008
Research Article | Open Access | Issue | Published: 11 September 2021

Health benefits of Grifola frondosa polysaccharide on intestinal microbiota in type 2 diabetic mice

Show Author's Information Xiaoxiang Gaoa,1,  Dan Liua,1,  Luying Gaob,  Yuezhen Ouyanga,  Yuxi Wena,  Chao Aic,d,  Yuqing Chena,  Chao Zhaoa,e( )
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Department of Pediatrics, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China
Department of Food Science & Technology, National University of Singapore, Singapore 117543, Singapore
Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou 350002, China

1 The authors contributed equally to this study.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

Keywords:
Gut microbiota, Diabetes, Maitake, Grifola frondosa polysaccharide
Cite this article:
Gao X, Liu D, Gao L, et al. Health benefits of Grifola frondosa polysaccharide on intestinal microbiota in type 2 diabetic mice. Food Science and Human Wellness, 2022, 11(1): 68-73. https://doi.org/10.1016/j.fshw.2021.07.008
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Abstract Full text About this article

Grifola frondosa polysaccharide (GFP) as the natural compounds have been reported to exert diverse bioactivities. The aim of this study was to investigate the regulatory effects of GFP on intestinal microbiota in type 2 diabetic mice. The changes of microbiota in faeces of the diabetic mice upon high fat diet were determined and the V3 region of the 16S rRNA was sequenced by Illumina MiSeq high-throughput sequencing platform. Eighty operational taxonomic unit were identified to be shared by all samples, and the quality and richness of sequencing were assessed via rarefaction and rank abundance curves. The diversity of gut flora was slightly improved in diabetic mice after GFP treatment. The composition and relative abundance of gut microbiota at phylum and genus levels were altered by GFP. The abundance of Robinsoniella, Flavonifractor, Anaerotruncus, and Desulfvibrio were found to concentrate on diabetic mice through LEfSe analysis. Furthermore, Alloprevotella and Burkholderia had strong relevancy with other gut flora. Based on the findings, GFP might be a desired candidate on ameliorating the intestinal unbalance in diabetes.


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About this article

Health benefits of Grifola frondosa polysaccharide on intestinal microbiota in type 2 diabetic mice

Show Author's information Xiaoxiang Gaoa,1, Dan Liua,1, Luying Gaob, Yuezhen Ouyanga, Yuxi Wena, Chao Aic,d, Yuqing Chena, Chao Zhaoa,e( )
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Department of Pediatrics, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China
Department of Food Science & Technology, National University of Singapore, Singapore 117543, Singapore
Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou 350002, China

1 The authors contributed equally to this study.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

Abstract

Grifola frondosa polysaccharide (GFP) as the natural compounds have been reported to exert diverse bioactivities. The aim of this study was to investigate the regulatory effects of GFP on intestinal microbiota in type 2 diabetic mice. The changes of microbiota in faeces of the diabetic mice upon high fat diet were determined and the V3 region of the 16S rRNA was sequenced by Illumina MiSeq high-throughput sequencing platform. Eighty operational taxonomic unit were identified to be shared by all samples, and the quality and richness of sequencing were assessed via rarefaction and rank abundance curves. The diversity of gut flora was slightly improved in diabetic mice after GFP treatment. The composition and relative abundance of gut microbiota at phylum and genus levels were altered by GFP. The abundance of Robinsoniella, Flavonifractor, Anaerotruncus, and Desulfvibrio were found to concentrate on diabetic mice through LEfSe analysis. Furthermore, Alloprevotella and Burkholderia had strong relevancy with other gut flora. Based on the findings, GFP might be a desired candidate on ameliorating the intestinal unbalance in diabetes.

Keywords: Gut microbiota, Diabetes, Maitake, Grifola frondosa polysaccharide

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Publication history
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Publication history

Received: 29 March 2020
Revised: 10 June 2020
Accepted: 16 June 2020
Published: 11 September 2021
Issue date: January 2022

Copyright

© 2021 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Acknowledgements

Acknowledgements

This work was financially supported by Double First-Class Construction Plan (KSYLX013) and Science and Technology Innovation Project (CXZX2017524) of Fujian Agriculture and Forestry University.

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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