568
Views
4
Downloads
161
Crossref
N/A
WoS
177
Scopus
0
CSCD
The present study was aimed to investigate the in vitro free radical scavenging activity of various leaf extracts (aqueous, methanol, acetone chloroform and petroleum ether) of Kedrostis foetidissima. In vitro free radical scavenging activities of the extracts were assessed against DPPH and hydroxyl radicals. The metal chelating activity and reducing power ability of the extracts were also determined. The free radical scavenging activity was found to be high in methanolic extract for DPPH and hydroxyl radicals in a concentration dependent manner followed by chloroform, aqueous, acetone and petroleum ether extracts. The metal chelating activity and reducing power ability was also found to be high in methanolic extract. The difference in scavenging potential of the extracts may be due to variation in the percentage of phytoconstituents extracted in various solvents. Thus the result suggests that the methanolic leaf extract of K. foetidissima could serve as a potential source of antioxidants and can be explored as a therapeutic agent in free radical induced diseases.
The present study was aimed to investigate the in vitro free radical scavenging activity of various leaf extracts (aqueous, methanol, acetone chloroform and petroleum ether) of Kedrostis foetidissima. In vitro free radical scavenging activities of the extracts were assessed against DPPH and hydroxyl radicals. The metal chelating activity and reducing power ability of the extracts were also determined. The free radical scavenging activity was found to be high in methanolic extract for DPPH and hydroxyl radicals in a concentration dependent manner followed by chloroform, aqueous, acetone and petroleum ether extracts. The metal chelating activity and reducing power ability was also found to be high in methanolic extract. The difference in scavenging potential of the extracts may be due to variation in the percentage of phytoconstituents extracted in various solvents. Thus the result suggests that the methanolic leaf extract of K. foetidissima could serve as a potential source of antioxidants and can be explored as a therapeutic agent in free radical induced diseases.
A. Tiwari, Imbalance in antioxidant defense and human diseases: multiple approach of natural antioxidants therapy, Curr. Sci. 81 (2001) 1179–1187.
D.A. Joyce, Oxygen radicals in disease, Adv. Drug React. Bull. 127 (1987) 476–479.
B. Halliwell, J.M. Gutteridge, Role of free radicals and catalytic metal ions in human disease: an overview, Methods Enzymol. 186 (1990) 1–88.
S.A. Stanner, J. Hugher, C.N. Kelly, J.A. Butlriss, Review of the epidemiological evidence for the antioxidant hypothesis, Public Health Nutr. 7 (2000) 401–422.
J.M. Braugghler, C.A. Duncan, L.R. Chase, The involvement of iron in lipid peroxidation: importance of ferrous to ferric ratio in initiation, J. Biol. Chem. 61 (1986) 102–182.
M.A. Anagnostopoulou, P. Kefalas, V.P. Papageorgiou, D. Boskou, Radical scavenging activity of various extracts and fractions of sweet orange peel (Citrus sinensis), Food Chem 94 (2006) 19–25.
H. Sagakami, T. Aohi, A. Simpson, S. Tanuma, Induction of immunopotentiation activity by a protein-bound polysaccharide PSK, Anticancer Res 11 (1991) 993–1000.
K.P. Tatjana, K. Svetlana, S. Marina, In vitro antioxidant activity of some Teucrium species (Lamiaceae), Acta Pharm. 55 (2005) 207–214.
B. Sangameswaran, D. Chubhale, B.R. Balakrishnan, B. Jayakar, Hepatoprotective effects of Thespesia lampas, Dhaka Univ. J. Pharm. Sci. 7 (1) (2008) 11–13.
K. Shimada, K. Fujikawa, K. Yahara, T. Nakamura, Antioxidative properties of xanthan on the autioxidation of soybean oil in cyclodextrin emulsion, J. Agric. Food Chem. 40 (1992) 945–948.
M. Oyaizu, Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine, Jpn. J. Nutr. 44 (1986) 307–315.
T.C.P. Dinis, V.M.C. Madeira, L.M. Almeida, Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers, Arch. Biochem. Biophys. 315 (1994) 161–169.
S.M. Klein, G. Cohen, A.I. Cederbaum, Production of formaldehyde during metabolism of dimethyl sulphoxide by hydroxyl radical generating system, Biochemistry 20 (1981) 6006–6012.
A.A. Dehpour, M.A. Ebrahimzadeh, S.F. Nabavi, S.M. Nabavi, Antioxidant activity of methanol extract of Ferula assafoetida and its essential oil composition, Grasas Aceites 60 (4) (2009) 405–412.
S.M. Nabavi, M.A. Ebrahimzadeh, S.F. Nabavi, M. Fazelian, B. Eslami, In vitro antioxidant and free radical scavenging activity of Diospyros lotus and Pyrus boissieriana growing in Iran, Pharmacognosy Mag. 4 (18) (2009) 123–127.
S. Meir, J. Kanner, B. Akiri, S.P. Hadar, Determination and involvement of aqueous reducing compounds in oxidative systems of various senescing leaves, J. Agric. Food Chem. 43 (1995) 1813–1817.