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Short Communication | Open Access

Development of the A&GBE base editor for efficiently generating abundant genetic variation in rice

Wenhui Zhang, Yaqi Xu, Jingxuan Cui, Yingshuang Xue, Lijie Zhang, Wenjuan Wu, Chong Wang, Hui Zhang( )
Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development, Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China
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Abstract

We have developed a dual base editor, rA&GBE, by fusing adenine and glycosylase base editors. It can induce up to eight types of mutations in T0-generation rice, including single-base conversion, simultaneous multiple-base conversions, and InDels, using a single guide RNA. A-to-G and C-to-G/T conversions occur simultaneously on the same DNA strand. The rA&GBE system may prove useful for crop improvement and in planta direct evolution.

Keywords

CRISPRA&GBEGenetic variationRice

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The Crop Journal
Volume 12 Issue 6,
December 2024
Pages 1801-1805
DOI: 10.1016/j.cj.2024.08.009

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Cite this article:
Zhang W, Xu Y, Cui J, et al. Development of the A&GBE base editor for efficiently generating abundant genetic variation in rice. The Crop Journal, 2024, 12(6): 1801-1805. https://doi.org/10.1016/j.cj.2024.08.009

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Received: 24 April 2024
Revised: 30 July 2024
Accepted: 26 August 2024
Published: 24 September 2024
© 2024 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).