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The functional assay of chemical sensitive cells is of great importance for the preparation of sensitive elements towards the development of cell-based biosensors for chemical sensing. In this study, a novel light-addressable microfluidic device was developed by the integration of a light-addressable potentiometric sensor (LAPS) with a microfluidic chip for label-free functional assays of bioengineered taste receptor cells via extracellular recording. Extracellular potential changes of single bioengineered cells were recorded by LAPS. Microfluidic chip was capable of providing stable microenvironments for cell measurements with a well-defined concentration stimulus. Bioengineered taste receptor cells were utilized as a model of chemical sensitive cells and functional assayed by this light-addressable microfluidic device using bitter stimulation. The results indicate that this microfluidic device can efficiently monitor the membrane potential changes originated from bioengineered taste receptor cells in response to specific bitter stimulation. The bioengineered cells with responsive functions can be easily identified via the analysis on the firing rates of extracellular recording data. The expression of bitter receptors in the bioengineered taste receptor cells was further validated by the immunofluorescent staining results, which proved that the expression of specific bitter receptor was the main contribution to the responsive function of bioengineered cells. This microfluidic device can not only be used for the functional assays of chemical sensitive cells towards chemical sensing, but also suitable to be applied for the research on the chemical signal transduction mechanisms.
Bashir R, (2004) BioMEMS: state-of-the-art in detection, opportunities and prospects.Adv Drug Deliv Rev 56:1565-1586
Chandrashekar J, Hoon MA, Ryba NJ, Zuker CS, (2006) The receptors and cells for mammalian taste.Nature 444:288-294
Dittrich PS, Manz A, (2006) Lab-on-a-chip: microfluidics in drug discovery.Nat Rev Drug Discovery 5:210-218
Du LP, Zou L, Zhao LH, Huang LQ, Wang P, Wu CS, (2014) Label-free functional assays of chemical receptors using a bioengineered cell-based biosensor with localized extracellular acidification measurement.Biosens Bioelectron 54:623-627
Du WB, Fang Q, He QH, Fang ZL, (2017) High-throughput nanoliter sample introduction microfluidic chip-based flow injection analysis system with gravity-driven flows.Anal Chem 77:1330-1337
Duffy DC, McDonald JC, Schueller OJ, Whitesides GM, (1998) Rapid prototyping of microfluidic systems in poly(dimethylsiloxane).Anal Chem 70:4974-4984
Gao J, Yin XF, Fang ZL, (2004) Integration of single cell injection, cell lysis, separation and detection of intracellular constituents on a microfluidic chip.Lab Chip 4:47-52
Garstecki P, Fuerstman MJ, Stone HA, Whitesides GM, (2006) Formation of droplets and bubbles in a microfluidic T-junction-scaling and mechanism of break-up.Lab Chip 6:437-446
Gilbertson TA, Boughter JD, Zhang H, Smith DV, (2001) Distribution of gustatory sensitivities in rat taste cells: whole-cell responses to apical chemical stimulation.J Neurosci 21:4931-4941
Hafeman DG, Parce JW, McConnell HM, (1988) Light-addressable potentiometric sensor for biochemical systems.Science 240:1182-1185
Ismail AB, Yoshinobu T, Iwasaki H, Sugihara H, Yukimasa T, Hirata I, Iwata H, (2003) Investigation on light-addressable potentiometric sensor as a possible cell-semiconductor hybrid.Biosens Bioelectron 18:1509-1514
Kimmerling RJ, Lee Szeto G, Li JW, Genshaft AS, Kazer SW, Payer KR, de Riba Borrajo J, Blainey PC, Irvine DJ, Shalek AK, Manalis SR, (2016) A microfluidic platform enabling single-cell RNA-seq of multigenerational lineages.Nat Commun 7:10220
Liu QJ, Wu CS, Cai H, Hu N, Zhou J, Wang P, (2014) Cell-based biosensors and their application in biomedicine.Chem Rev 114:6423-6461
Mark D, Haeberle S, Roth G, von Stetten F, Zengerle R, (2010) Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications.Chem Soc Rev 39:1153-1182
Matsunami H, Montmayeur JP, Buck LB, (2000) A family of candidate taste receptors in human and mouse.Nature 404:601-604
Mazutis L, Gilbert J, Ung WL, Weitz DA, Griffiths AD, Heyman JA, (2013) Single-cell analysis and sorting using droplet-based microfluidics.Nat Protoc 8:870-891
Miyamoto K, Itabashi A, Wagner T, Schöning MJ, Yoshinobu T, (2014) High-speed chemical imaging inside a microfluidic channel.Sens Actuators B 194:521-527
Owicki JC, Bousse LG, Hafeman DG, Kirk GL, Olson JD, Wada HG, Parce JW, (1994) The light-addressable potentiometric sensor: principles and biological applications.Annu Rev Biophys Biomol Struct 23:87-113
Pancrazio JJ, Whelan JP, Borkholder DA, Ma W, Stenger DA, (1999) Development, application of cell-based biosensors.Ann Biomed Eng 27:697-711
Richards Grayson AC, Shawgo RS, Johnson AM, Flynn NT, Li YW, Cima MJ, Langer R, (2004) A bioMEMS review: MEMS technology for physiologically integrated devices.Proc IEEE 92:6-21
Stein B, George M, Gaub HE, Parak WJ, (2004) Extracellular measurement of averaged ionic currents with the light-addressable potentiometric sensor (LAPS).Sens Actuators B 98:299-304
Suzuki H, Yoneyama R, (2003) Integrated microfluidic system with electrochemically actuated on-chip pumps and valves.Sens Actuators B 96:38-45
Wang J, Du LP, Krause S, Wu CS, Wang P, (2018) Surface modification and construction of LAPS towards biosensing applications.Sens Actuators B 265:161-173
Wise KD, Najafi K, (1991) Microfabrication techniques for integrated sensors and microsystems.Science 254:1335-1342
Wu CS, Chen P, Yu H, Liu QJ, Zong XL, Cai H, Wang P, (2009) A novel biomimetic olfactory-based biosensor for single olfactory sensory neuron monitoring.Biosens Bioelectron 24:1498-1502
Wu CS, Du LP, Zou L, Huang LQ, Wang P, (2013) A biomimetic bitter receptor-based biosensor with high efficiency immobilization and purification using self-assembled aptamers.Analyst 138:5989-5994
Wu CS, Du LP, Zou L, Zhao LH, Huang LQ, Wang P, (2014) Recent advances in taste cell- and receptor-based biosensors.Sens Actuators B 201:75-85
Wu CS, Du Y-W, Huang LQ, Galeczki YB, Dagan-Wiener A, Naim M, Niv M, Wang P, (2017) Biomimetic sensors for the senses: towards better understanding of taste and odor sensation.Sensors 17:2881
Xu GX, Ye XS, Qin LF, Xu Y, Li Y, Li R, Wang P, (2005) Cell-based biosensors based on light-addressable potentiometric sensors for single cell monitoring.Biosens Bioelectron 20:1757-1763
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